Biocontrol MVP ICON - User Manual

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Surface Sampling Device

GENERAL DESCRIPTION

The LIGHTNING MVP ICON Surface Sampling Device is a

self-contained device for use with the LIGHTNING MVP

ICON™ or LIGHTNING MVP

systems for quality and

HACCP-related monitoring of the environment. The MVP

ICON and LIGHTNING MVP systems detect food and

microbiological residues by measuring Adenosine

triphosphate (ATP) using luciferase bioluminescence. ATP is

found in most food residues and all bacteria, yeast and mold

cells.

Part No. 64003-100 (100 tests)

KIT COMPONENTS

One box of 100 surface sampling devices

IMPORTANT USAGE GUIDELINES

Do not insert wet or contaminated sampling devices into the

instrument. Wipe condensate from exterior of device before

use with a clean, lint-free cloth. Avoid handling the device

with wet or contaminated hands.

Always remove sampling devices from instrument after

reading.

Do not store the MVP ICON or MVP instrument on its side

or back. Avoid storing in humid areas where water leaks are

possible.

TEST PROCEDURE

A. Collecting Sample

(a) Twist to remove sampling device from body tube. The

swab tip must be moist. If fully saturated, any excess

wetting agent should be blotted off by gently pressing the

swab tip against the interior of the body tube. Be careful

not to touch the swab or inside the sampling device with

fingers.

(b) Thoroughly swab a standard 10 x 10 cm (4 x 4 in) area of

interest for a typical flat surface. For irregularly shaped

surfaces, ensure swabbing technique remains consistent

each time you swab. Reinsert the swab into the body

tube using a twisting motion to ensure it is sealed.

Note: After swabbing, the sampling device is stable for one

hour prior to activation.

B. Activating Device and Reading Results

(a) To activate the sampling device, hold upright and push

the plunger down until it is fully depressed. The reagents

will be dispensed through the sampling device washing

the sample off the swab tip and into the collection tube.

(b) Insert the sampling device into the MVP ICON or MVP

and read the results. When inserting into the MVP

ICON, ensure the flat sides of the sampling device base

chamber face left-to-right in the instrument.

Note: Results should be read within two minutes of

activation. Please refer to the MVP ICON User Guide or

MVP System Guide for operating instructions.

INTREPRETING RESULTS

(a) When using default thresholds in the MVP ICON or MVP

systems, readings of 2.5 Zones or less indicate the

surface is considered clean.

(b) Readings between 2.6 - 3.0 Zones indicate a warning the

surface is not adequately clean.

considered dirty and should be re-cleaned and re-tested.

CONTROLS

A. Positive Control

It is recommended LIGHTNING MVP ICON ATP Positive

Controls (Part No. 64001-25) be run every other day to

verify integrity of the reagent system.

(a) A positive control should yield a result from 3.1 to 4.3

Zones. See ATP Positive Control Directions for Use for

additional information.

B. Negative Control

A negative control is an unused yet activated LIGHTNING

MVP ICON Surface Sampling Device.

(a) Negative control results should read between 1.0 to 2.5

Zones.

(b) Readings above 2.5 may indicate contamination of the

MVP ICON or MVP sampling chamber or sampling

device.

See ATP Positive Control Directions for Use for additional

information. Contact BioControl Technical Support at

800.245.0113 for more information.

PRECAUTIONS

The appearance of a yellow band on the swab tip is normal

and will not affect performance.

If accidental activation prior to sampling occurs, do not use

device.

Hold sampling device upright when activating.

Read sampling device within two minutes of activation.

When reading the sampling device, the MVP ICON or MVP

must be kept upright (no greater than 45° angle). See MVP

ICON User Guide or MVP System Guide for additional

information.

STORAGE

Sampling devices should be stored at a temperature between

2 ºC – 30 ºC (36 ˚F – 86 ºF). Protect from direct sunlight.

Unused devices must be resealed in the foil bags.

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Summary

General Information and Usage

General Description

Overview of the LIGHTNING MVP ICON Surface Sampling Device and its purpose in environmental monitoring.

Kit Components

Lists the items included in the product kit.

Important Usage Guidelines

Key instructions for handling and storing the sampling devices to ensure proper function and accuracy.

Test Procedure

Collecting Sample

Step-by-step guide on how to collect a surface sample using the MVP ICON device.

Activating Device and Reading Results

Instructions for activating the sampling device and obtaining results from the MVP ICON instrument.

Results Interpretation and Controls

Interpreting Results

Guidelines for understanding the zone readings and assessing surface cleanliness based on results.

Positive Control

Procedure and expected results for running a positive control to verify reagent system integrity.

Negative Control

Procedure and expected results for running a negative control to check for contamination.

Precautions and Storage

Precautions

Important safety and operational notes to prevent device damage or inaccurate readings.

Storage

Recommended temperature and conditions for storing the sampling devices to maintain viability.

Warranty Information

Warranty

Details the product warranty, including terms, conditions, and limitations of liability.