Document # 49044IM1
Revision 7.5 21
Dated February 16, 2017
APPENDIX A
1. Ensure the Two Water Cuvettes Match
Follow this procedure if the tracings are > 4% from 0% or 100%
on the graph.
a. After wiping the outside with a Kim Wipe
®
, place BLACK
cuvette in the PRP well of Channel 1 and a Water
cuvette in the PPP well with the alignment mark placed
left center.
b. Click on the Channel 1 “Activate” bar.
c. Push and Hold the Set Baseline Button until the tracing
reaches a full 100% of graph before releasing. Tracing
will then return to 0% of graph.
d. After wiping the outside with a Kim Wipe
®
, replace the
BLACK cuvette with the 2
nd
Water cuvette from the kit,
with the alignment mark placed left center. The
tracings should move near 100% on the graph. Allow
to run for a few seconds and note the position where
the tracing stabilizes. (Ex: 98%)
e. Keeping the alignment marks in the left center
position, swap the cuvettes (ie: take the cuvette from
the PRP well and place it in the PPP well. Take the
cuvette from the PPP well and place it in the PRP well).
Note the position where the tracing stops (Ex: 97%)
f. If the difference between the two numbers (98% and
97%), is < 4%, the two Water cuvettes are matched
and can be used to check the calibration and/or to
perform Calibration.
g. If the difference between the two numbers is > 4%, the
two Water cuvettes are not matched and should be
replaced.
h. Contact Chrono-log to order P/N 322 Calibration Kit
containing (1) BLACK Cuvette and (2) sealed Water
Cuvettes.
NOTE: If it is determined that Calibration cannot be delayed to
wait for delivery of a new Kit, standard P/N 312 cuvettes can be
substituted, as described in Step 2.
2. Ensure P/N 312 Water Cuvettes Do Not Contain any Major
Scratches/Flaws
a. Make sure that the cuvettes are clean and there are no
fingerprints on the bottom half. Different cuvettes will
give slightly different outputs, so visually inspect
several cuvettes and pick three (3) cuvettes that
appear to be most free from blemishes. The fewer
blemishes in the cuvette the more accurate the
calibration.
b. Take the three (3) new cuvettes and pipette 500 µL of
water into each. Incubate the cuvettes for a minimum
of three (3) minutes in the incubation wells.
c. After the three (3) minute incubation, check cuvettes
for air bubbles. Eliminate bubbles by tapping the
cuvette.
d. After wiping the outside with a Kim Wipe
®
, place BLACK
cuvette in the PRP well of Channel 1.
e. Using a Kim Wipe
®
, wipe the outside of (1) prewarmed
P/N 312 cuvette containing water and place in the PPP
well of Channel 1.
f. Press Set Baseline. Be sure tracing reaches a full 100%
of graph before releasing.
g. With tracing stable at 0%, slowly turn the Water
cuvette in the PPP well and look for any large spikes
[> 5%] in the tracing. If there are large fluctuations,
discard the cuvette and repeat with another. If there
are no large fluctuations, turn the cuvette so the
tracing returns to 0% line and mark the top left center
of the cuvette with a dark Alignment Mark.
h. Repeat steps 5 thru 7 above until there are 3 marked
cuvettes.
i. Follow Instructions in 1. above to select (2) matched
Water cuvettes.
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