Document # 49044IM1
Revision 7.5 19
Dated February 16, 2017
c. Push SELECT pushbutton for Channels 1 and 2 to return
to Default screen … which will inactivate the SET
pushbutton for those two channels.
d. Repeat steps a through c for remaining channels.
2. Under the AGGREGOMETER window, select or set-up a Test
Procedure page for Optical mode. Click on OK.
3. Select RUN NEW PATIENT under AGGREGOMETER and Click
on OK.
4. After wiping the outside with a Kim Wipe® , place BLACK
cuvette in Channel 1 PRP well and the PPP water cuvette in
the PPP well of Channel 1, with the alignment mark placed
left center.
5. Click on the Channel 1 “Activate” bar. [NOTE: If this test is to
be SAVED, Click on the “Start” Bar for Channel 1. If not
saving, the following steps can be performed after clicking
on the “Activate” button.]
6. Push and Hold the Set Baseline button until the tracing
reaches a full 100% of graph before releasing. Tracing will
then return to 0% of graph.
7. After wiping the outside with a Kim Wipe®, replace the
BLACK cuvette in the PRP well with the PRP water cuvette.
The tracing should move near 100% on the graph. Allow to
run for a few seconds and note the position where the
tracing stabilizes. (Ex: 97%).
8. Repeat Steps 4 through 7 for remaining channels, except …
leave the PPP cuvette in the Channel 1 PPP well. Also be
sure to place the Alignment Mark to the left center in each
test well and to wipe the outside of the cuvettes with a Kim
Wipe® when placing into the test well.
9. If any tracing is > 4% from 0% or 100% on the graph,
calibration of that Channel will need to be repeated,
following the previous calibration steps.
10. If saving the test … Once all channels are completed, Click on
the STOP icon followed by the SAVE icon and the PRINT icon
to save and print this test.
NOTE: If the tracings are > 4% from 0% or 100% on the graph,
this may indicate that Auto-Calibration was not set properly.
Recheck the two water cuvettes to be sure they match. See
Appendix A for further instructions on matching water cuvettes,
then repeat the entire calibration procedure. Be sure to wipe the
cuvettes with a KimWipe® each time they are placed into a test
or reference well. If, after the second attempt, the results are
still > 4%, the system may need service. Contact Chrono-log
Service Department at 1-800-247-6665 for further assistance.
Discussion
The Black cuvette blocks all light from passing through. The clear
water samples allow the maximum amount of light through. Each
instrument is adjusted at the factory for a set voltage with water
samples. The automatic calibration procedure resets the voltage
while the water samples are in the wells.
The water samples have equal optical density and should allow
the same amount of light to pass through the samples. This will
result in a tracing at 100% of the test graph. This tracing is used
to check that the calibration is set properly.
Sometimes the difference is caused by variations in the cuvettes.
If the baseline is not within 4% of the 100% baseline, the P/N 322
Kit may need to be replaced.
The PRE-calibration, Calibration and POST- Calibration steps
described above will catch calibration errors, including conditions
where one channel has matching wells that do not match the
remaining channels. Calibration with empty wells is one instance
that would create this problem. Faults may occur at any time due
to spills, broken glass, etc. Daily Quality Control checks can be
performed to prevent false readings.
If the tracing is still > 4% after following this procedure, contact
Chrono-log Service Department at 1-800-247-6665 to inquire
about instrument service.
Troubleshooting Guide
Many factors will affect the performance and reproducibility of
test runs on the Aggregometer. These can be broken down into
several categories; Instrument failure or Calibration problems,
Sample and Materials problems, and Operator technique related
problems. Below is a brief list of common problems.
Missing stir bar
Bubbles in sample blocking optical path
Sample volume too low
Platelet count- PRP below 75K, PPP above 3K
Platelet count- mismatch between PPP samples for each
Cuvette dirty, scratched
Stirring RPM setting mismatch
Stirring motor failure
Inconsistent pipetting; force, position, tip too short.
ROUTINE QC CHECKS should be performed on a quarterly basis, or
if any of the following conditions should exist during testing.
Optical calibration should be checked with Water Samples as
described under the Pre-Calibration procedure. If required,
Calibration of the Optical Circuits can be performed using the
same Water Samples as described under Calibration.
Test Results > 100%
Test Results on all channels do not fall within +/- 10% of the
average
If these suggestions do not solve the problem, then contact
Chrono-log or your local distributor to inquire about service.
Assistance and Distributor Information
For Assistance on training, service, software updates and other
information, contact your Chrono-log representative or your local
distributor. Information on Chrono-log Distributors can be found
on the Chrono-log website at www.chronolog.com.
To Next Page
Loading...