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• Use quartz or UV transparent cuvettes for UV range measurements.
• Ensure cuvettes are clean prior to use.
• Fill cuvettes to manufacturer's specifications.
• Confirm the mode selection has been changed on the Run screen to the proper
cuvette pathlength prior to a measurement.
• Ensure cuvettes are properly inserted following the etched arrow light path.
• Refer to a reagent kit manufacturer for troubleshooting colorimetric assays.
Fluorescence Mode
• Use 200 μL volumes in a clear thin walled PCR tube
• Avoid introducing air bubbles into the sample solution when mixing samples.
• Ensure all sample concentrations in the thin walled assay tubes fall within the
limits of the assay or reagent kit for accurate results.
• Follow assay reagent manufacturers' recommendations regarding temperature,
incubation time, and protection from ambient light.
• Follow assay reagent manufacturers' recommendations regarding suggested
standard curve concentrations and data analysis.
• Confirm that the correct LED excitation source was used for all user-defined
assays and methods.
• Confirm that standard concentrations and dilutions were performed correctly.
• Confirm that the correct concentration units for the standard curve and the
unknown samples were used to calculate the stock concentrations.
Software Errors
Pop-up messages will provide explanations for software-based errors or invalid
user actions.
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