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M
mallory10Jul 28, 2025
Why are washes after fixation important for diagenode Bioruptor?
- DDavid WatsonJul 28, 2025
Washing the fixed cells properly is important. Make sure to eliminate all the formaldehyde. Glycine can be used to stop the fixation.
Why are washes after fixation important for diagenode Bioruptor?
Washing the fixed cells properly is important. Make sure to eliminate all the formaldehyde. Glycine can be used to stop the fixation.
How long should the fixation step be for diagenode Bioruptor Medical Equipment?
The fixation should be done for 10 minutes, but a time course can be used when needed.
What temperature should I use for fixation with diagenode Bioruptor Medical Equipment?
Fixation should be done at room temperature.
What is the formaldehyde final concentration for diagenode Bioruptor Medical Equipment?
The formaldehyde final concentration is 1%.
General| Model | Bioruptor |
|---|---|
| Application | DNA, Chromatin, and Protein Shearing |
| Technology | Ultrasonication |
| Number of Samples | 12 tubes per run |
| Cooling | Requires water cooling system (e.g., Diagenode Cooling System) |
| Programmable Cycles | Yes |
| Timer | Yes, programmable timer |
| Voltage | 100-240 VAC |
| Type | Ultrasonic cell disrupter |
General overview and safety conditions for installing the equipment.
How to program cycle number, ON/OFF times, and intensity.
Recommended protocol and operating conditions for DNA shearing.
Specifies sample volume, tubes, buffer, and concentration for DNA shearing.
Guidance on choosing tubes and optimal sample volumes.
Advice on DNA concentration, sample preparation, and viscosity.
Importance of DNA quality, purity, and extraction methods.
How water temperature affects sonication and cooling methods.
Guidance on sonication timing and sonication bath upkeep.
Key factors affecting chromatin shearing efficiency and protocol.
