Sampling
User Manual Biosen C_line Clinic / GP+
6. Sampling
The sample is taken up with a 20µl end-to-end capillary and hemolyzed
in a micro test tube, pre-filled with a hemolyzing solution. This involves
thinning the sample by 1:51. Capillary blood, venous blood, arterial
blood, plasma or serum can be used as sample material
Sampling errors directly lead to erroneous measurement
results. Always observe the following instructions.
For sample collection, you should have ready an opened prefilled micro
test tube, a capillary, and possibly a capillary holder (see Section 16,
page 54).
When opening and closing the micro test tube, take caution so as not to
splash or spill the solution.
6.1 Taking a sample of capillary blood
With poor peripheral circulation, glucose concentrations
in capillary blood may deviate greatly from concentrations in venous or
arterial blood. Examples are critically ill patients, shock, severe
hypotension, severe dehydration or hyperosmolar hyperglycemia. In
these cases measurement of glucose from capillary blood is not clinically
recommended and should be done from venous or arterial blood
i,ii
.
Risk of infection, wear suitable gloves!
Atkin SH, Dasmahapatra A, Jaker MA, Chorost MI, Reddy S., Fingerstick glucose
determination in shock, Ann Intern Med. 1991 Jun 15;114(12):1020-4.Links
Desachy A, Vuagnat AC, Ghazali AD, Baudin OT, Longuet OH, Calvat SN, Gissot V.
Accuracy of bedside glucometry in critically ill patients: influence of clinical characteristics
and perfusion index, Mayo Clin Proc. 2008 Apr. 83(4):400-5.
Reaction sample caps and hemolysis solutions must be
combined with the Measurement Control Mode (see Section 7.1.1,
page 24).
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