GE HEALTHCARE AKTAprime plus User Manual (English)

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Questions and Answers:

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robert41Aug 3, 2025
What to do if there is noisy UV-signal, signal drift or instability in GE HEALTHCARE AKTAprime plus Laboratory Equipment?
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  Wesley HernandezAug 3, 2025
  If you observe a noisy UV-signal, signal drift, or instability in your GE HEALTHCARE Laboratory Equipment, several factors could be at play. First, the UV cell might be dirty; try cleaning it by flushing it with Decon™90, Deconex™11, or an equivalent solution. Another potential cause is an impure buffer. To check this, see if the signal remains noisy when using water. Finally, air in the pump or UV cell could be the culprit. In this case, purge the pump as described in the Pump User Manual and run a system wash with buffer.
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harrisonjenniferAug 13, 2025
How to fix ghost peak in GE HEALTHCARE AKTAprime plus?
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  Patricia EllisAug 13, 2025
  If you are experiencing ghost peaks with your GE HEALTHCARE Laboratory Equipment, it could be due to a few reasons. There might be dirt or residues in the flow path from previous runs, or air in the eluents. To resolve this, clean the system and ensure all air is removed. Another cause might be residue in the column from previous runs, so clean the column according to its instructions. Also, the mixer function could be incorrect. Verify the mixer function by placing a stirrer bar on top of the mixer housing; it should rotate when the system is in Run mode. You can also check the mixer function by running the installation test.
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Colleen WatsonAug 22, 2025
Why does GE HEALTHCARE Laboratory Equipment have low sensitivity?
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  Sarah RossAug 22, 2025
  Low sensitivity in your GE HEALTHCARE Laboratory Equipment can stem from a few potential issues. One common cause is an aging UV lamp. Check the lamp's run time and replace it if necessary, referring to the ÄKTAprime plus User Manual. Another possibility is that the UV lamp is in the wrong position. Ensure that both the lamp and filter positions are set to the correct wavelength (280 nm or 254 nm), as detailed in the ÄKTAprime plus User Manual. Lastly, the theoretical extinction coefficient might be too low. Calculate the protein's theoretical extinction coefficient; if it is zero or very low at 280 nm, the protein cannot be detected.