ÄKTAprime plus
GE Healthcare
Cue Cards
Contents
System Preparation 2
Buer exchange on HiTrap Desalting 4
Buer exchange on HiPrep 26/10 Desalting 6
Histidine-tagged protein purication,
step elution 8
Histidine-tagged protein purication,
gradient elution 10
IMAC purication - any metal 12
On-column refolding 14
GST-tagged protein purication 16
Strep(II)-tagged protein purication 18
MBP-tagged protein purication 20
MAb purication, step elution 22
MAb purication, gradient elution 24
IgM purication 26
Albumin removal 28
Removal of trypsin-like serine proteases 30
Anion exchange 32
Cation exchange 34
Method Templates value table 36