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Ettan DALTsix
GE HEALTHCARE Ettan DALTsix User Manual
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Ettan DALT
six
Electrophoresis System
User Manual
GE Healthcare
806492
49AC
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1
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13,
2006
2:
42 P
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Table of Contents
Table of Contents
5
1 Ettan Daltsix Electrophoresis System Function and Description
7
System Function and Description
7
Electrophoresis Unit
7
Gel Caster
7
Gel Casting Cassettes
8
Gradient Maker
8
2 Specifications
9
Ettan Daltsix Electrophoresis Unit
9
3 Preparing the Ettan Daltsix Gel Caster
11
4 Casting Homogeneous Gels
13
5 Casting Gradient Gels
15
Preparation for Gradient Gel Casting
15
Gradient Casting Setup
15
Pouring Gel Solutions for Gradient Gels
16
6 Unloading the Gel Caster
19
7 Using Ettan Daltsix for Electrophoresis
21
Preparing Second Dimension Gels: Equilibration and Loading
21
Preparing the Electrophoresis Unit
22
Unloading the Gels and Cleaning the Unit
24
Recommended Running Conditions
24
8 Recipes
25
Volume Required for (Ml)
26
Final %T 10% 12.5
26
Acrylamide Stock 1.5 M Tris-Cl, Ph
26
Water 10% SDS 10% APS 10% TEMED
26
Recipes 8
27
Volume Required for (Ml) Final %T 8% 10% 12% 14
27
Acrylamide Stock
28
Water
28
10% Sds
28
10% Aps
28
10% Temed
28
Volume Required for (Ml)
28
Glycerol
28
9 Troubleshooting
29
10 Care and Maintenance
33
Cleaning
33
Replacement of Components
33
Recycling
33
11 Customer Service Information
35
Technical Service and Repair
35
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GE HEALTHCARE Ettan DALTsix Specifications
General
Brand
GE HEALTHCARE
Model
Ettan DALTsix
Category
Medical Equipment
Language
English
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