14
COLLECTION MODES | FC 203B FRACTION COLLECTOR
COlleCtiOn mOdes
In peak modes, the fraction collector needs the following
information:
• the width of a typical peak in the run at half of its
maximum height, measured in minutes
• the height of the shortest peak of interest, measured
in millivolts (this number is easily determined from
the full scale voltage deflection of the detector and
recorder) or the millivolt (mV) level above which peaks
will be collected
• the time it takes for the effluent to pass from the
detector flow cell to the dispense head of the fraction
collector. This aids in the collection of purer peaks. The
user’s guide explains how to calculate the delay time
based on the setup.
Optionally, collect the non-peak effluent at a different
time/tube or drop/tube than the peak effluent.
To discard non-peak effluent to waste, press the
Discard Soft Key. When you discard non-peak effluent, it
is highly recommended that you use a 3-way valve. Refer
to the user’s guide.
Parameters:
Peak: _ _ . _ _ min/tube
or
Peak: _ _ _ _ drops/tube
Non-peak: _ . _ _ min/tube
or
Non-peak: _ _ _ _ drops/tube
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