1-40
GENERAL DIGESDAHL DIGESTION, continued
8. Take the flask off
the heater and allow
the flask to cool.
Remove the
fractionating column
from the digestion
flask.
Note: Use finger cots to
remove the digestion
flask. Place it on a
cooling pad for at least
one minute. Then
remove the column.
REQUIRED REAGENTS
Quantity
Description Per Digestion Unit Cat. No.
Hydrogen Peroxide, 50%. . . . . 10 mL . . . . . . . 500 mL . . . . .21196-49
Potassium Hydroxide
Standard Solution, 1 N . . . varies . . . . . . . . 59 SCDB*. . . 23144-26
Potassium Hydroxide
Standard Solution, 8 N . . . . varies . . . . . . . . 500 mL . . . . . . . 282-49
Sulfuric Acid, ACS (concentrated,
specific gravity 1.84) . . . . . ≥3 mL . . . . . . . 4 kg . . . . . . . . . .979-09
Water, deionized . . . . . . . . . . . varies. . . . . . . . 3.78 L . . . . . . . .272-17
REQUIRED APPARATUS
Dispenser, pour-out, 10 mL. . . 1. . . . . . . . . . . . each. . . . . . . . 22200-38
Pipet, serological, 10 mL. . . . . 1. . . . . . . . . . . . each. . . . . . . . . . 532-38
Pipet Filler, safety bulb . . . . . . 1. . . . . . . . . . . . each. . . . . . . .14651-00
Boiling Chips,
silicon carbide. . . . . . . . . . . varies . . . . . . . . 500 g . . . . . . .20557-34
7. Boil off excess
hydrogen peroxide by
heating for one more
minute after addition
of hydrogen peroxide
is complete. Do not
heat to dryness.
Note: If the sample goes
to dryness, turn off the
Digesdahl and cool
completely. Add water
to flask before handling.
Repeat digestion from
the beginning.
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