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illumina iScan - User Manual

illumina iScan
80 pages
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For Research Use Only. Not for use in diagnostic procedures.
August 2015
Document # 11313539 v01
Material # 20000396
Catalog # SY-950-1001DOC
ILLUMINA PROPRIETARY
iScan System Guide

Questions and Answers

  • N
    Nathan GomezSep 12, 2025
    What to do if illumina All in One Printer iScan Control Software (ICS) displays FPGA timeout errors?
    • J
      Jack GriffinSep 12, 2025
      Take a screen capture of the error and save it. Close the iScan Control Software and power cycle the scanner to clear the FPGA. Restart the iScan Control Software to see if the error repeats. If the error occurs again, contact Illumina Technical Support and schedule a field service call. This can happen because of problems with emission filter slide, excitation filter wheel, tilt motor, tray switch, and/or laser safety switch.
  • M
    Mary HillAug 23, 2025
    Why are the status lights not functioning properly during scanning with my illumina iScan All in One Printer?
    • F
      felicia01Aug 23, 2025
      If the status lights spontaneously turn on or off during a run and no error message appears in the iScan Control Software, continue the run through completion, and then reinitialize the iScan Reader. If the problem persists, contact Illumina Technical Support. The cause might be an environmental electrostatic discharge affecting the status lights.
  • D
    Darlene BeckAug 25, 2025
    What does it mean if the illumina All in One Printer iScan Control Software displays x-motor, y-motor, or z-motor errors?
    • R
      Rebecca FreemanAug 25, 2025
      A motor error occurred in an x-, y-, or z-stage motor. Sometimes an error occurs for one motor because of an initial error in another motor. Take a screen capture of the error and save it. If the error caused the scan to stop, close the iScan Control Software and power cycle the scanner to rehome the motors. Restart the iScan Control Software to see if the error repeats. If the error occurs again, contact Illumina Technical Support and schedule a field service call. If the BeadChip does not lie flat or is improperly seated within a carrier, eject the BeadChip carrier and examine how the carrier is seated in the iScan Reader adapter tray. Try reseating the BeadChips within the carrier and restarting the scan.
  • J
    Jesse SmithAug 26, 2025
    Why does a section appear slightly compressed and distorted on my illumina All in One Printer?
    • L
      Leah PhillipsAug 27, 2025
      The appearance of the section has no effect on your data. Use the horizontal and vertical size controls of your monitor to manipulate the appearance of a section so it appears regular (all sides are the same length). Make sure that your video driver resolution is set to 1280 x 1024. This can happen if your monitor is not adjusted for displayed resolution.
  • P
    Peter HillAug 27, 2025
    How to fix cannot connect to iScan Reader error on illumina All in One Printer?
    • B
      Barbara HicksAug 27, 2025
      Inspect the cable between the iScan Reader and the instrument control computer to confirm that the cable is securely connected at both ends. If the cable between iScan Reader and PC might be unplugged. Remove any external hard drive or other USB device before turning on the iScan Reader and its computer, allowing the override.cfg file to be loaded on the internal drive H if there is an initialization failure error when starting the iScan Control Software.
  • D
    Dawn SandersSep 18, 2025
    Why are buttons inaccessible or text/icons distorted on my illumina iScan?
    • K
      Kerri WeberSep 18, 2025
      Set monitor resolution to a minimum of 1280 x 1024, and 16 bit color. This is because the monitor resolution might be too low.
  • J
    Justin ContrerasSep 23, 2025
    Why does my illumina All in One Printer computer displays blue screen?
    • D
      Dr. Sarah WiseSep 23, 2025
      Check to see if the camera link cable leading to the frame grabber card has become loose. If the cable seems tight, reseat the frame grabber card, if there is a loose cable connection between camera and frame grabber card. Contact Illumina Technical Support to schedule a field service call, if a large number of ports have been installed on the computer.
  • B
    baxterscottSep 10, 2025
    What to do if illumina iScan is able to autoalign, but unable to scan sections successfully?
    • B
      brenda13Sep 12, 2025
      Contact the responsible scientist to discuss the history of the assay preparation for the BeadChips and the expectation of the scientist. Evaluate the image output of nearby images using images stored in the workspace for this BeadChip. If the issue is not resolved, contact Illumina Technical Support. This can happen if problems during BeadChip preparation can result in some sections appearing too dim to perform registration and bead intensity extraction.
  • J
    John HughesSep 6, 2025
    What does it mean if the fault light illuminates on my illumina All in One Printer?
    • M
      Melissa GonzalezSep 6, 2025
      Click the Menu button in the upper left corner of the iScan Control Software screen, and then select Scanner | Initialize. This is because the iScan Reader requires reinitialization.
  • G
    gregorybrownSep 7, 2025
    What does it mean when the reader initialized LED displays yellow or red on illumina iScan All in One Printer?
    • M
      Morgan HunterSep 7, 2025
      Reinitialize the iScan Reader and iScan Control Software. For more information, see Starting the iScan System on page 13, if iScan Reader does not initialize correctly. Reinitialize the iScan Reader and iScan Control Software, and perform a power cycle on the computer. If the scanner still fails to initialize correctly, contact Illumina Technical Support, if iScan Reader does not initialize correctly after cycling instrument power and restarting iScan Control Software.

Summary

Starting the iScan System

Powering up the iScan System

Details the procedure for powering on the iScan Reader and control computer.

Starting the iScan Control Software

Explains how to launch and initialize the iScan Control Software.

Loading BeadChips onto the iScan

Loading BeadChips onto a Carrier

Details the process of placing beadchips into a carrier slot.

Loading a Carrier into the iScan Reader

Explains how to insert a loaded beadchip carrier into the iScan Reader tray.

Configuring iScan Control Software

Changing Scan Settings

Details creating and applying custom scan settings files.

Scanning Procedures

Starting a Scan

Describes the prescan steps and initiation of the scanning process.

Viewing Scan Results

Images

Details how to review and adjust scanned beadchip images.

Shutting Down, Maintenance, and Service

Shutting Down the iScan System

Provides step-by-step instructions for safely shutting down the iScan system.

Troubleshooting the iScan System

Handling Errors

Explains how the system records and reports errors for technical support.

Registration Issues

Addresses problems with IDAT file creation and image registration.

Auto-Alignment Issues

Covers problems related to the autoalignment process.

iScan Reader Issues

Troubleshoots connectivity, status lights, and beadchip detection problems.

illumina iScan Specifications

General IconGeneral
Brandillumina
ModeliScan
CategoryAll in One Printer
LanguageEnglish