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Leica DM6000 B User Manual

Leica DM6000 B
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Leica DM6000B
Leica DM6000M
Operating Manual

Table of Contents

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Leica DM6000 B Specifications

General IconGeneral
TypeResearch microscope
Optical SystemInfinity Optics
Objective LensesPlan Apo, HC PL Fluotar, and other Leica objectives
IlluminationLED or halogen illumination
StageMotorized stage
CameraOptional digital camera integration
SoftwareLeica Application Suite (LAS)
FocusingMotorized focus drive with fine and coarse adjustment.
Contrast MethodsBrightfield, darkfield, phase contrast, fluorescence
NosepieceRevolving nosepiece with multiple objective positions
ControlIntegrated control panel or software control
Eyepieces10x or 12.5x
CondenserAbbe condenser
DimensionsVaries depending on configuration
WeightApproximately 20 kg
AutomationFully automated, including objective change, focus, illumination, and stage movement.

Summary

Important Notes about this Manual

Text symbols, pictograms and their meanings

Explains the meaning of various text symbols, pictograms, and their graphical representations used in the manual.

Intended Purpose of Microscopes

Safety Notes

General Safety Notes

Provides general safety instructions and compliance information for the device and its accessories.

Electrical Safety

Details electrical safety specifications and precautions for various components like the CTR6000 Electronics Box.

Overview of the Instrument

Unpacking the Microscope

Assembling the Microscope

Stage

Instructions for installing and adjusting the microscope stage, including safety notes.

Condenser

Steps for mounting and connecting the condenser, with notes on centering.

Tube and Eyepieces

Guide for attaching the tube and inserting eyepieces, including motorized tube connection.

Objectives

Information on objective receptacles and recommendations for parfocality adjustment.

Light Sources for the Transmitted Light Axis

Procedure for installing lamp housings and connecting the power supply for transmitted light.

Light Sources for the Incident Light Axis

Instructions for installing and connecting the 106 z lamp housing for incident light.

Equipping the Incident Light Turret Disc

Guide on how to insert filter and reflector cubes into the incident light turret.

Polarizer and Analyzer

Instructions for assembling the ICT/P transmitted light polarizer and incident light polarizers.

Optional Accessories

Information on connecting cameras and optional accessories like c-Mounts and Vario-Mounts.

Connecting the Leica CTR6000 Electronics Box

Details on connecting the CTR6000 Electronics Box to the microscope and its components.

Connecting the Computer

Instructions for connecting the computer to the microscope via the RS232C port.

Connection to the Power Supply

Steps for connecting the CTR6000 electronics box and ebq 100 supply unit to the power source.

Startup

Functional Principle

Describes the intelligent automation concept and control elements of the Leica DM6000 B/M.

Switching on

Procedure for switching on the microscope and its components, including initialization phase.

The LeicaScreen

Explanation of the LeicaScreen's display, navigation panel, menu bar, and control panel.

The Function Keys at the stand

Details on defined and variable function keys located on the microscope stand.

The Remote Control Element SmartMove

Information on turning knobs and variable function keys for stage and focus control.

Köhler Illumination

Procedure for setting up Köhler illumination for transmitted light and incident light.

Checking Phase Contrast Rings

Guidance on checking and centering phase contrast rings for objectives.

Adjusting Motorized Polarizer

Steps for adjusting the motorized polarizer to achieve maximum darkness.

Adjusting the Light Sources

Procedures for adjusting transmitted and incident light sources, including lamp centering.

Operation

Switching on

Procedure for powering on the microscope and its external supply units.

Stages and Specimen Displacement

Instructions for moving the stage, setting step sizes, and storing/recalling stage positions.

Focusing

Guide to focusing the image, setting thresholds, and parfocality adjustment.

Tubes

Details on adjusting tube settings, viewing distance, viewing angle, and beam splitting.

Eyepieces

Instructions for adjusting eyepieces, including those with inlaid reticles and vision correction.

Objectives

Guidance on selecting objectives, changing operation modes (DRY/IMM), and handling immersion objectives.

Magnification Changer

Information on using the magnification changer and setting magnification factors.

Light Sources

Instructions for setting brightness using function keys and INT keys for different light sources.

Aperture Diaphragm and Field Diaphragm

Procedure for adjusting aperture and field diaphragms using function keys.

Contrasting Methods for Leica DM6000 B

Transmitted Light

Overview of transmitted light contrasting methods like Bright Field, Phase Contrast, Dark Field, Polarization, and DIC.

Bright Field (TL)

Procedure for setting up and using the Bright Field contrast method for transmitted light.

Phase Contrast

Instructions for using the Phase Contrast method, including objective selection and diaphragm settings.

Dark Field (TL)

Guide for setting up and using Dark Field contrast, including aperture limits.

Polarization (TL)

Steps for implementing Polarization contrast using manual or motorized procedures.

Differential Interference Contrast (TL)

Procedure for using DIC contrast, including manual adjustments for polarizer and analyzer.

Fluorescence

Instructions for setting up and using fluorescence, including filter cube selection and intensity adjustment.

Combi Mode

Information on using Combi modes (FLUO/PH, FLUO/DIC) and switching between light axes.

Contrasting Methods for Leica DM6000 M

Incident Light

Overview of incident light contrasting methods like Bright Field, Dark Field, Polarization, DIC, and Fluorescence.

Bright Field (IL)

Procedure for setting up and using Bright Field contrast for incident light.

Dark Field (IL)

Guide for setting up and using Dark Field contrast for incident light.

Polarization (IL)

Steps for implementing Polarization contrast for incident light using manual procedures.

Interference Contrast (IL)

Procedure for using DIC contrast for incident light, including manual adjustments.

Fluorescence

Instructions for setting up fluorescence for incident light, including filter cube selection.

Transmitted Light

Overview of transmitted light contrasting methods like Bright Field and Polarization.

Bright Field (TL)

Procedure for setting up and using Bright Field contrast for transmitted light.

Polarization (TL)

Steps for implementing Polarization contrast for transmitted light using manual or motorized procedures.

Trouble Shooting

Care of the Microscope

Dust Cover

Instructions on using the dust cover to protect the microscope and its accessories.

Cleaning

Guidelines for cleaning coated parts, lenses, and the stage, with precautions for chemicals.

Handling Acids and Bases

Precautions for using acids and bases, emphasizing protection of optics and mechanical parts.

Essential Wear and Spare Parts

Abbreviations and Pictograms

Index

EU Declaration of Conformity

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