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Malvern Nano ZS90 ZEN3690 User Manual

Malvern Nano ZS90 ZEN3690
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Zetasizer Nano Series
User Manual
Zetasizer Nano Series
User Manual
MAN0317 Issue 1.0 June 2003

Table of Contents

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Malvern Nano ZS90 ZEN3690 Specifications

General IconGeneral
ModelZEN3690
Temperature Range0°C - 90°C
Light SourceHe-Ne laser
DetectorAvalanche Photodiode (APD)
Scattering Angle90°
Detector Angle173°
SoftwareZetasizer Software
Particle Size Range0.3 nm to 10 µm
Zeta Potential Range±200 mV
Molecular Weight Range980 Da to 20 MDa
Concentration Range0.1 mg/mL
Laser Type633 nm
Sample Volume12 μL
Cell TypesDisposable cuvettes
Measurement TypeDynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS)

Summary

What is the Zetasizer Nano?

Zetasizer Nano Capabilities

Explains the instrument's ability to measure particle size, zeta potential, and molecular weight.

Measurement Principles: Size, Zeta, Molecular Weight

Defines and explains the fundamental concepts of particle size, zeta potential, and molecular weight measurements.

Making Measurements: A Tutorial

Quick Guide to Measurement

Provides a brief overview of the steps involved in performing a measurement using an SOP.

Sample Preparation and Cell Handling

Covers essential steps of sample preparation, choosing, filling, and inserting the correct measurement cell.

Measurement Procedures (SOP & Manual)

Explains how to perform measurements using Standard Operating Procedures (SOPs) or manually.

Measurement Display and Result Editing

Describes the measurement display interface and how to re-analyze and edit obtained results.

Sample Preparation

Preparing Samples for Size Measurement

Outlines basic considerations for preparing samples for size measurements, focusing on concentration.

Preparing Samples for Molecular Weight

Details specific considerations and procedures for preparing samples for molecular weight analysis.

Preparing Samples for Zeta Potential

Covers concentration requirements, dilution, and medium considerations for zeta potential measurements.

Using Standard Operating Procedures (SOPs)

Creating a New SOP

Guides users through the step-by-step process of creating a new Standard Operating Procedure using the wizard.

Specific SOP Types (Size, MW, Zeta)

Details the unique measurement dialogues specific to creating SOPs for Size, Molecular Weight, and Zeta Potential.

Measurement File and Workspace Management

Workspace Customization

Explains how to customize window views using workspaces for specific measurement types and data display.

Exporting Measurement Results

Creating Custom Reports

Size Theory

Dynamic Light Scattering (DLS) Principles

Explains the core principles of Dynamic Light Scattering (DLS) and how it measures particle size.

Zetasizer Nano Size Measurement Operation

Details the physical operation of the Zetasizer Nano for performing size measurements, including optics and detection.

Molecular Weight Theory

Static Light Scattering (SLS) Principles

Explains the principles of Static Light Scattering (SLS) for determining molecular weight.

Zeta Potential Theory

Understanding Zeta Potential and the Double Layer

Explains zeta potential, the electrical double layer, and factors affecting it like pH.

M3-PALS Technique for Zeta Potential

Details Malvern's patented M3-PALS technique for accurate zeta potential measurements, avoiding electroosmosis.

Zeta Potential Measurement Operation

Describes the physical operation of the Zetasizer Nano for zeta potential measurements.

APPENDIX A Health and Safety

General Safety Regulations

Provides general warnings and regulations for safe operation of the system.

Electrical and Laser Safety

Outlines electrical safety precautions and laser safety information for the instrument.

Temperature, Purge, and Sample Handling Warnings

Covers warnings related to cell temperature, purge supply, and safe handling of samples and dispersants.

APPENDIX C Site Requirements

Environmental and Space Requirements

Specifies the required environmental conditions and physical space for operating the Zetasizer Nano.

APPENDIX E Installation

Installing the Zetasizer Nano System

Provides step-by-step instructions for connecting the instrument and computer.

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