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Malvern Zetasizer Nano Series User Manual

Malvern Zetasizer Nano Series
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Zetasizer Nano Series
User Manual
Zetasizer Nano Series
User Manual
MAN0317 Issue 1.0 June 2003

Table of Contents

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Malvern Zetasizer Nano Series Specifications

General IconGeneral
Measurement PrincipleDynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS)
DetectorAvalanche Photodiode (APD)
SoftwareZetasizer Software
Measurement TypeParticle Size, Zeta Potential, Molecular Weight
Particle Size Range0.3 nm to 10 μm
Molecular Weight Range980 Da to 20 MDa
Temperature Range0°C to 90°C
Light Source633 nm He-Ne laser
Minimum Sample Volume12 μL
Sample Volume Range12 μL to 1 mL

Summary

Chapter 1 - Introduction to the Zetasizer Nano

How to Use This Manual

Explains the manual's structure, purpose, and how to navigate its sections.

Access to the Instrument

Details authorized personnel and their access levels to the Zetasizer instrument.

Where to Get Help

Provides guidance on obtaining assistance from the manual and the support help desk.

Chapter 2 - Understanding the Zetasizer Nano

What Does the Zetasizer Nano Do?

Explains the core measurement capabilities: particle size, zeta potential, and molecular weight.

The Zetasizer Nano Range

Details the different models within the Zetasizer Nano particle analyser series.

What is Particle Size, Zeta Potential and Molecular Weight?

Provides basic explanations of the key measurement parameters.

Chapter 3 - Zetasizer Nano Operation and Components

How is a Zetasizer Measurement Performed?

Gives an overview of the measurement process, system components, and software functions.

Identifying the Hardware

Identifies the key hardware modules of the Zetasizer instrument and their functions.

Navigating the Software

Describes the key features and navigation of the main Zetasizer Nano software application.

Chapter 4 - Making Measurements: A Tutorial

Quick Guide to Making a Measurement

Provides a brief overview of the measurement process using an SOP.

Powering Up the System

Details the steps for powering up the Zetasizer instrument and starting the software.

Sample Preparation

Emphasizes the importance of sample preparation for accurate measurements.

Choosing the Correct Cell

Discusses the selection of appropriate cells based on measurement type and sample.

Filling the Cell

Provides guidance on the correct procedure for filling various types of cells.

Making an SOP Measurement

Details the process of performing a measurement using a Standard Operating Procedure (SOP).

Making a Manual Measurement

Explains how to perform a measurement by manually setting parameters.

The Measurement Display

Describes the features and functions of the measurement display window.

Editing the Result

Explains how to re-analyze measurement records with different parameters.

Chapter 5 - Records and Reports - Viewing Results

Introduction

Explains how to review final measurement results displayed as records or reports.

Records View

Describes how measurement records are listed and displayed in the measurement file window.

Report Tabs - A Typical View

Explains how to select and view measurement information through report tabs.

System Details

Details instrument settings like temperature, count rate, cell type, and attenuation index.

Results

Explains the interpretation of measurement results, including Z-average size and Polydispersity Index.

Chapter 6 - Sample Preparation

Introduction

Emphasizes the importance of proper sample preparation for reliable measurements.

Preparing the Sample - Size

Outlines considerations for sample preparation concerning particle size and concentration.

Preparing the Sample - Molecular Weight

Details specific considerations for preparing samples for molecular weight analysis.

Preparing the Sample - Zeta Potential

Outlines concentration requirements and dilution procedures for zeta potential measurements.

Chapter 7 - Maintenance

Introduction

States that maintenance is minimal and outlines user-performed procedures.

Cleaning the Instrument

Provides instructions for cleaning the external surfaces of the instrument safely.

Cleaning the Cells

Details procedures for cleaning Universal 'Dip' cells and cuvettes.

Replacing the System Fuse

Explains how to replace system fuses and their specifications.

Chapter 8 - Security

Introduction

Explains the Zetasizer's security system for limiting user access and controlling functions.

Initial Start-Up - Set Up the Administrator

Guides on setting up the administrator account for system configuration.

Enabling Security

Details the process of enabling the security system within the software.

User Groups

Explains how to manage user groups and their associated permissions.

Chapter 9 - Using SOPs

Introduction

Explains the benefits and methods of using SOPs for consistent measurements.

Creating an SOP

Details the steps and dialogues involved in creating a new SOP.

All SOPs

Describes common dialogues used across all SOP types for measurement setup.

Size SOPs

Details specific measurement dialogues unique to size measurements within SOPs.

Molecular Weight SOPs

Details specific measurement dialogues unique to molecular weight measurements within SOPs.

Zeta Potential SOPs

Details specific measurement dialogues unique to zeta potential measurements within SOPs.

Trend & Protein Melting Point SOPs

Explains how to set up SOPs for trend and protein melting point measurements.

Extracting an SOP

Describes how to view and save an SOP from an existing measurement record.

Modifying an SOP

Explains how to edit and save existing SOPs.

Chapter 10 - Measurement File Window and Workspace Management

Introduction

Introduces the Measurement File window for viewing and analyzing results and reports.

Measurement File Window

Describes how to open and navigate the measurement file window containing records and reports.

Workspaces

Explains how to customize window views by creating and selecting workspaces.

Handling Measurement Files

Covers methods for moving, copying, deleting, sorting, and printing measurement records.

Chapter 11 - Exporting Results

Introduction

Explains the capabilities of the Zetasizer software for exporting data to external applications.

Exporting Results

Guides on how to export measurement files, records, or selections using various methods.

Creating an Export Template

Describes how to create and modify templates for defining data export parameters.

Chapter 12 - Creating Custom Reports

Introduction

Introduces Malvern's default reports and the use of Report Designer for custom reports.

Opening a Report

Details how to open existing reports or create new ones using Report Designer.

Customising and Editing the Report

Explains how to customize properties of text, parameters, pictures, frames, and calculations in reports.

Viewing the New Report

Explains how to associate and view newly created custom reports within the Zetasizer software.

Chapter 13 - Size Theory

Introduction

Outlines the chapter's aim to describe size principles and Dynamic Light Scattering.

What is Dynamic Light Scattering?

Explains DLS as a technique for measuring particle size via Brownian motion and scattered light.

Operation of the Zetasizer Nano - Size Measurements

Details the physical operation of size measurements using the Zetasizer Nano system.

Chapter 14 - Molecular Weight Theory

Introduction

Outlines the chapter's aim to describe molecular weight principles and Static Light Scattering.

What is Static Light Scattering?

Explains SLS as a non-invasive technique for characterizing molecules in solution.

The Debye Plot

Explains the Debye plot and its use in determining molecular weight and the 2nd Virial Coefficient.

Chapter 15 - Zeta Potential Theory

Introduction

Outlines the chapter's aim to describe zeta potential principles and measurement operation.

What is Zeta Potential?

Defines zeta potential and the electrical double layer around particles.

The M3-PALS Technique

Details the M3-PALS technique for analyzing low mobility samples.

Operation of the Zetasizer Nano - Zeta Potential Measurements

Details the physical operation of zeta potential measurements.

Appendix A - Health and Safety

General Warnings and Safety Regulations

Covers general hazards, proper system usage, and environmental conditions.

Electrical Warnings and Safety Regulations

Highlights warnings related to mains power, high voltage components, and electrical safety.

Laser Safety Regulations

Details laser safety classifications and precautions, noting Class 1 product status.

Sample Handling Warnings

Provides guidance on safe handling of samples and dispersants according to regulations.

Appendix B - Specification

Specification

Lists the technical specifications of the Zetasizer Nano system and its components.

Appendix C - Site Requirements

Introduction

Outlines the necessary site requirements for installing the Zetasizer Nano system.

Environmental Conditions

Specifies the required environmental conditions for operating the instrument and accessories.

Space Required

Details the recommended space allocation for system components and access.

Power Requirements

Lists the power requirements for the Zetasizer Nano, Autotitrator, and computer.

Computer Specification

Outlines the minimum PC specifications required for running the Zetasizer software.

Appendix D - Unpacking Instructions

Unpacking Instructions

Provides step-by-step instructions for safely unpacking the Zetasizer Nano instrument.

Appendix E - Installation

Introduction

Explains how to re-install the Zetasizer Nano system after moving or changing computers.

Installing the Zetasizer Nano

Details the simple steps for connecting the Zetasizer Nano instrument to a computer.

Changing the Computer

Outlines the actions required when replacing the computer used with the instrument, including software setup.

Appendix F - Regulatory Statements

CE Declaration of Conformity

States the product's conformance to European Commission Directives.

Federal Communications Commission (FCC) Notice

Provides FCC compliance information and operating conditions for the device.

Canadian Regulatory Information

Lists Canadian regulatory compliance information for digital apparatus.

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