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NANOSIGHT NS300
Malvern NANOSIGHT NS300 Operating Manual
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NANOSIGHT NS300
OPERATING MANUAL
MAN0516-08-EN-00 January 2016
2
Table of Contents
Default Chapter
1
Operating Manual
1
Table of Contents
3
Introduction
5
Explanation of Warning Symbols
5
Important Notes
5
Safety
5
Laser Safety
5
Electrical Safety
6
General Safety
6
Servicing
7
Maintenance
7
Handling
7
Unpacking and Initial Inspection
8
Installation and Relocation
8
Returning Equipment
8
Warranty
9
2 Nanosight Ns300 Hardware
10
Ns300 Technical Specifications
10
Ns300 Instrument Housing
10
Ns300 Laser Module
10
Laser Classification
10
Ns300 Setup
11
Instrument Communications
11
Laser Module Configuration
11
Top-Plate Selection
11
External Fluidic Setup
12
3 Low Volume Flow Cell
13
System Setup
13
Flow Cell Assembly
13
Flow Cell Tubing Connection
14
Flow Cell Usage
15
Priming Tubing
15
Changing Inlet Tubing Syringe
15
Loading an Initial Sample
15
Changing Samples
16
Manual Cleaning Procedures
17
Drying the Low-Volume Flow Cell
18
Care and Maintenance
19
Gasket Component Replacement
19
4 O-Ring Top Plate
20
System Set-Up
20
O-Ring Top-Plate Assembly for Manual Injection
20
O-Ring Top-Plate Assembly for Syringe Pump Use
20
O-Ring Syringe Pump Tubing Connection
21
O-Ring Top-Plate Usage
21
Manual Injection of a Sample
21
Loading a Sample for Use with a Syringe Pump
22
Priming Tubing
22
Changing Inlet Tubing Syringe
22
Loading the Sample through the Syringe Pump Tubing
23
Changing Samples
24
Manual Cleaning Procedures
24
Drying the O-Ring Top-Plate
25
Care and Maintenance
25
5 Mounting the Laser Module
26
6 Daily Use
26
7 Nanosight Ns300 Control Software
27
Nta Software Startup
27
Camera Setup
28
Adjusting the Ns300 to Obtain a Video
28
Thresholding Pixels (High-Sensitivity Systems Only)
29
Fluorescence Mode
29
Viewing Fluorescently Labelled Particles
29
Capture Videos
30
Temperature Control
30
Operation
30
Initialising
31
Temperature Setting
31
8 Spare Parts List
32
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Malvern NANOSIGHT NS300 Specifications
General
Brand
Malvern
Model
NANOSIGHT NS300
Category
Measuring Instruments
Language
English
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