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General| Brand | Micromass |
|---|---|
| Model | Quattro Ultima |
| Category | Laboratory Equipment |
| Language | English |
Symbol indicates the presence of high voltages on the instrument.
Symbol indicates that the instrument has hot surfaces.
Symbol indicates user should consult the guide to prevent instrument damage.
Warnings for personal injury avoidance and proper instrument use.
Physical dimensions of the instrument with a diagram.
Weight specifications for the instrument, data system, and pumps.
Warnings and guidelines for safe lifting and handling of the instrument.
Electrical voltage and current specifications for the instrument and pumps.
Ambient temperature, heat dissipation, and humidity limits.
Specification for heat dissipation into water for cooling the system.
Requirement to vent rotary pump exhaust to atmosphere via fume hood or vent.
Venting API gas exhaust to atmosphere; caution about connection to rotary pump exhaust.
Specification for dry, oil-free nitrogen supply and tubing requirements.
Specification for Argon as collision gas and required pressure.
Overview of the Quattro Ultima MS-MS system and its coupling capabilities.
Description of ion flow through source, quadrupoles, and detector system.
Details of rotary and turbomolecular pumps used for vacuum generation.
Vacuum measurement gauges and system interlocks for protection.
Description of APcI mechanism, sample vaporization, and flow rates.
Description of ESI mechanism, charged droplets, and flow rates.
Nanoflow interface for low flow rate ESI and sensitivity gains.
How samples are introduced via liquid pumping systems and capillaries.
Explanation of MS1 and MS2 modes for basic MS analysis.
Summary of four MS-MS scan functions: Daughter Ion, Parent Ion, MRM, Constant Neutral Loss.
Typical applications like structural elucidation and method development.
Example spectrum illustrating daughter ion analysis.
Applications for structural elucidation and complementary data.
Example spectrum illustrating parent ion analysis.
MRM for high sensitivity screening and applications.
Example of MRM transition monitoring and chromatogram output.
How the spectrum is generated and what it represents.
Role of PC data system and MassLynx NT in instrument control.
Connection of PTFE gas lines for desolvation and nebuliser gases.
Electrical connections for ESI capillary, APcI discharge pin, and ESI/APcI heaters.
Description of Vacuum and Operate LEDs indicating instrument status.
Table detailing states and indications for the Vacuum LED.
Table detailing states and indications for the Operate LED.
Needle valves for gas control and caution for CID Gas valve.
Optional valve for injection or switching solvent flow, pneumatically operated.
Outputs for peripheral connection, switchable voltage or contact closure.
Inputs for external devices to start sample acquisition.
Analog inputs for simultaneous data acquisition from detectors.
6-way connector for instrument to MUX control base.
RJ45 connector for instrument to data system via network cable.
Water usage for turbomolecular pump cooling.
Connection for nitrogen supply, tubing requirements, and caution.
Requirements for venting rotary pump and gas exhausts; caution on combining lines.
Requirements for power cord connection and mains switch function.
Fuse information and rotary pump control via data system.
Connection for grounding to prevent electrostatic discharge.
Location and quantity of power supplies for various instrument components.
Diagram showing location of Transputer, Analogue, Control, Scan Control, and RF Control PCBs.
Details on low/high voltage power supplies and RF generators.
Functions of Pumping Logic PCB and Power Sequence PCB.
Functions of Transputer Processor Card, Analogue PCB, and Control PCB.
Functions of Scan Control PCB and RF Generator Control PCBs.
Description of source housing, analyser housing, and detector assembly.
Details on turbomolecular pumps, Pirani, and Active Inverted Magnetron gauges.
Location of the air filter at the front of the instrument.
Preparing instrument after inactivity: checking oil, connecting gases.
Verifying connections for rotary pump control, data system, and mains supplies.
Checking exhaust connections and switching on the instrument.
Steps for switching on the data system and launching the tune page.
Initiating pump down, monitoring vacuum status, and gas ballast valve operation.
Monitoring analyser pressure using Penning gauge and gauge life caution.
Starting instrument after overnight standby, checking vacuum LED.
Steps for fitting/removing corona discharge pin and connecting gas lines.
Connecting probe, setting source/desolvation temps, launching tune page.
Warnings for operating source without enclosure and handling hot components.
Steps for installing APcI corona discharge pin and connecting gas lines.
Connecting APcI probe, setting source temperature, launching tune page.
Warnings about operating source without enclosure and hot components.
Steps to enter operate mode via MassLynx, setting gas flows and temperatures.
Instrument protection against vacuum faults like pump malfunction, pressure, or temperature.
System response to pressure surge: voltage shutdown and amber Operate LED.
Consequences of pump fault: stop pumping, flashing red Vacuum LED, extinguished Operate LED.
Steps for power failure and subsequent restart.
Reference to Tuning section and specific tuning for ionisation techniques.
Reference to Mass Calibration section.
Reference to Data Acquisition and MassLynx NT User's Guide.
Worked example for daughter ion acquisition: tuning, parent ion selection, fragmentation.
Importance of accurately finding parent ion mass for daughter ion analysis sensitivity.
Adjusting Mass and Span, using Argon as collision gas.
Adjusting Entrance, Collision, Exit, and collision gas pressure.
Steps for immediate shutdown: power off and LC isolation.
Leaving instrument unattended: switching off LC pumps and nitrogen gas.
Steps for extended shutdown: venting, exiting software, power off.
Procedure for draining rotary pump oil if instrument is off for over a week.
Location and naming of automatic startup and shutdown files used by MassLynx.
Accessing and modifying automatic startup/shutdown procedures.
Options for immediate shutdown on error, after batch, or after time delay.
Defining sequences of operations within the Auto Control Tasks page.
Procedures for adding, inserting, and modifying tasks within the shutdown editor.
Methods for deleting individual or all tasks from the shutdown editor.
Mapping toolbar buttons to menu equivalents and their purpose.
Steps to open existing startup or shutdown files using the open file dialog.
Procedures for saving startup/shutdown files, including new and overwrite options.
How to print startup and shutdown files using the print dialog.
How to create a new startup or shutdown file using the New option.
Procedures for running and stopping automatic startup and shutdown files.
Importance of tuning and calibration before data acquisition for mass accuracy.
Adjusting parameters in Source and Analyser menus, optimizing collision energy.
How to display the tune page and print tune information.
Tuning parameters stored in data files and saving/restoring parameter settings.
Saving current tuning parameters to a file, including overwriting existing files.
Procedure to restore saved tuning parameters from a file.
Using Peak Editor to select peaks and adjust Mass, Span, and Gain.
Methods for changing tune mass and range using mouse or direct entry.
How to change the span of a peak using mouse or direct entry.
Methods for adjusting peak gain by double-clicking or direct entry.
Using popup menu to change peak colors and customize plot appearance.
Changing number of visible traces and adjusting color interpolation.
Options for displaying peak outline, fill, or min/max data points.
Options for relative/absolute intensity, normalized data, and grid lines.
How to run AutoTune for APcI/ESI modes and define setup parameters.
Explanation of full vs. maintenance AutoTune and parameter initialization.
Checks by AutoTune: parameter definition, readbacks, beam detection, focus, ion energy, resolution.
How to select the required ionisation mode from the Ion Mode menu.
Controlling scan time and inter-scan delay for tune peak display updates.
Turning gases on/off and setting up cone voltage ramps.
Setting up a collision energy ramp by defining values at two masses.
Repositioning zero or baseline by reinitialization to compensate for offsets.
Options for displaying readbacks continuously, hidden, or only when differing from defined values.
Typical voltages for ES/APcI probes, sample cone, and ion tunnels.
Typical voltages for RF Lens, Aperture, and Ion Energy.
Single sample from tune page, multiple samples from MassLynx screen.
Easiest way to acquire data, allowing parameter control and start/stop.
Parameters for data file naming, directory, and project creation.
Types of acquisition functions: MS, MS2, Daughter, Parent, Neutral Loss, Neutral Gain.
Data formats (Centroid, Continuum, MCA) and specifying mass range (Start/End Mass).
Using MassLynx sample list editor for quantitative analysis.
Steps to start multi-sample acquisition, including sample list dialog options.
Running tasks before/after acquisition (e.g., switching off gases).
Using Quantify Samples dialog for integration, calibration, and quantification.
Options for integrating samples, calibrating standards, quantifying, printing reports.
Setting the project for data acquisition and defining the sample range for analysis.
Acquisition status shown on MassLynx screen, MS panel, and status bar.
Displaying scan-by-scan statistical reports of acquisition progress.
Viewing chromatogram in real-time during acquisition via Display menu.
Viewing spectrum in real-time during acquisition via Display menu.
Parameters controlling data pre-processing before sending to computer.
Specifying data types for acquisition/discarding, and parameter download.
MaxEnt requirements and controls for profile data (Baseline Level, Points per Dalton).
Settings for centroid data (Minimum centroid height) and SIR data (SIR Baseline Level).
Setting intensity level below which data points are ignored, applied to all acquisitions.
Guidance on setting Ion Counting Threshold values for optimal noise removal.
How spikes are distinguished and removed by averaging neighbours.
Parameters for spike removal: Use Spike Removal, Min Spike Intensity, Spike Percentage Ratio.
Spike removal processing impact on acquisition rates and parameter settings.
Setting number of samples per second for analog data.
Checking communications status between MassLynx software and the embedded PC.
Methods to halt an acquisition from the tune page or MassLynx screen.
Purpose of function list editor for setting up scan sequences during acquisition.
Example of a simple function list with time scale display.
Example of a complex list with multiple SIR functions and selection.
Toolbar buttons for creating, opening, saving, and printing function lists.
How to add new functions using toolbar buttons or the Function menu.
Steps to modify existing functions or copy them using edit menu options.
Procedures for removing functions and changing their order in the list.
How to set a solvent delay period to prevent solvent peaks from eluting.
Acquiring and storing analog data from external units like UV detectors.
Procedures for saving current function lists, including overwriting existing files.
How to restore previously saved function lists from file.
Setting start/end mass and start/end time for acquisition.
Using tune page cone voltage and specifying ionization mode and data type.
Explanation of Centroid, Continuum, and MCA data types and their storage.
Scan Time, Inter-Scan Delay, and APcI Probe Temperature settings.
Editor for entering masses, dwell times, and inter-channel delay times for SIR.
Entering up to 32 masses to monitor, with dwell and cone values.
Double-clicking to display values, changing Mass, Dwell, Cone, and sorting.
Inter Channel Delay, Repeats, Span, and Retention Window (Start/End) settings.
Setting up Daughter scan using MS1 for parent mass and MS2 for fragment mass range.
Setting up Parent scan using MS1 to scan and MS2 for fragment mass.
Looking for the parent of a fragment, scanning MS1 over a range.
MS2 mode for resolving, Neutral Loss for detecting specific neutral fragments.
Specifying collision energy in electron volts for the collision cell.
Setting up a collision energy ramp by defining values at two masses.
Setting up MRM functions similar to SIR, monitoring MS-MS transitions.
Using Survey scans to search for precursor ions.
Pages for setting parameters for MS and MS-MS scanning during survey.
Criteria for switching from MS to MSMS based on TIC or Intensity thresholds.
Automatic, Include Masses Only, and Include Masses and Automatic precursor selection.
Auto exclude, Always include, and Include after time options.
Discarding uninteresting survey scans to save disk space.
Default method or After Time method for stopping MSMS functions.
Defining when MS scanning resumes based on conditions and threshold values.
Entering mass ranges and individual masses to include or exclude from MS-MS scans.
Display showing precursors currently running during an acquisition.
Overview of calibration process, including ES and APcI calibration.
Reference to calibration solutions and their preparation.
How MassLynx NT performs automated calibration using reference compounds.
Static, scanning, and scan speed compensation calibrations for MS1 and MS2.
Steps: Tuning, selecting reference file, starting automatic calibration, checking report.
Sensible to calibrate over a wide mass range using NaI/RbI for ES.
Using injector loop or infusion pump for reference compound introduction.
Setting Multiplier, source/ion energy parameters for unit mass resolution.
Checking parameters like data acquisition mode and scanning speeds.
Setting Profile Data to Compressed and 16 Points per Dalton for low mass calibration.
Selecting Calibration > Calibrate Instrument from the tune page.
Selecting reference files and removing current calibrations to ensure a clean state.
Setting limits for missed peaks and standard deviation for successful calibration.
Options for Span Correction and Acquisition Calibration Ranges checks.
Peak Match parameters (window, error) and Intensity threshold for data inclusion.
Selecting polynomial order (1-5) for calibration curve fitting, with recommendations.
Settings for Mass Measure when continuum or MCA data are acquired for calibration.
Adjusting centroiding parameters for high scan speeds to maintain resolution.
Selecting static, scanning, and scan speed compensation for MS1 and MS2.
Checking Acquire & Calibrate and Acquire & Verify options for calibration process.
Checking all calibration types (Static, Scanning, Scan Speed Comp.) for MS1 and MS2.
Selecting options in the Process area to perform and report the calibration.
Configuring mass range, run time, and data type for calibration.
Setting scan parameters and ensuring adequate run duration.
Setting static span size around reference peaks and dwell time.
Determining scan speeds for scanning calibration and scan speed compensation.
Order in which calibration files (STATMS1, SCNMS1, FASTMS1 etc.) are acquired.
Display of status messages and printed calibration reports upon completion.
Plots for MS1 Static, Scanning, and Scan Speed calibrations.
Plots for MS2 Static, Scanning, and Scan Speed calibrations.
Accessing calibration reports to view spectra and mass differences.
Examining peak matching and highlighting matched peaks in expanded regions.
Causes: no peaks, missed peaks, incorrect mode, threshold/window settings, wrong file.
Checking report for missed peaks and identifying potential causes.
Checking peak matches and deciding to accept or cancel the calibration.
Parameters leading to incorrect matching: threshold, error, window, std deviation.
Strategies for dealing with contamination peaks affecting peak matching.
Excluding or including peaks manually via the calibration report.
Saving a fully calibrated instrument state for future recall.
Performing verification instead of full recalibration, checking scanning acquisition.
Accessing dialogs, setting parameters, and starting verification procedure.
Display of verification results without changing instrument calibration.
Understanding PEG spectrum, doubly charged ions, and effects of ammonium acetate/cone voltage.
Complete mass calibration using APcI and reference compound preparation.
Procedure requires multiple steps due to short residence time of reference solution.
Using injection loop and solvent delivery system for APcI reference introduction.
Accessing options, selecting reference files, and removing current calibrations.
Recommending Polynomial order 2 for Curve Fit in APcI calibration.
Setting up static calibration for MS1, checking Acquire & Calibrate.
Default parameters for APcI reference file (pegh1000.ref), including mass range and data type.
Acquiring data using STAT file name, injecting reference solution.
Advisability of checking calibration manually whether successful or failed.
Using chromatogram window and Process, Combine Spectra to determine scan numbers.
Selecting Static calibration type and MS1, entering scan ranges, displaying report.
Referring to Calibration Failure section for peak matching issues.
Printing the calibration report from the report display.
Selecting OK from the calibration report to accept the calibration.
Acquiring two data files at slowest and fastest speeds for scanning calibration.
Setting Scan From/To, Scan Time, Inter Scan Delay, and Data Type.
Selecting Scanning type and MS1, entering start/end scans from report.
Comparing acquired and reference spectra to ensure correct peak matching.
Procedure using fast scanning file (FASTMS1) for scan speed compensation.
Performing calibration for MS2 by acquiring data in MS2 mode instead of MS1.
Judging calibration success by examining on-screen report; no warning message.
Reasons for failure: no peaks, missed peaks, incorrect mode, threshold settings, wrong reference file.
Checking report for missed peaks and identifying causes like low threshold or window settings.
Dealing with contamination peaks affecting peak matching and potential solutions.
Excluding or including peaks manually via the calibration report.
Saving calibrated states for different ionisation modes or future recall.
Setting up scanning acquisition and accessing calibrate dialog for verification.
Setting peak matching parameters and selecting verification options.
Display of verification results without changing instrument calibration.
Description of Z-spray source, electrospray probe, and optional nanoflow interface.
How mobile phase becomes aerosol spray, and applications for various analytes.
Common methods for delivering sample, including injection valve use.
Using infusion pump, mobile phase composition, and solvent degassing.
Method for checking flow rate from solvent delivery system and monitoring back pressure.
Recommendation to split flow post-column to approximately 200 µl/min for saving samples.
Adjusting split ratio by back pressure in waste line, and incorporating UV cell.
Enabling flow rates from 200 µl/min to 1 ml/min using microbore columns.
Using correct tubing (1/16" o.d., 0.007" i.d. peek) for megaflow operation.
Ensuring source assembly, nitrogen supply, and exhaust liner are correctly fitted.
Importance of exhaust liner and baffle for optimal intensity and stability at low flow rates.
Connecting probe to pump, checking liquid flow at tip, and turning on nitrogen.
Adjusting probe tip for complete nebulisation and checking capillary alignment.
Changing ionisation mode, setting desolvation gas flow, liquid flow, and temperature.
Optimizing parameters using a representative analyte sample.
Adjusting probe position (in/out, sideways) for optimal sensitivity and stability.
Further details on probe position adjustment and considerations for higher liquid flow rates.
Optimizing nebulisation by fully opening Nebuliser valve and adjusting Desolvation Gas flow.
Table relating solvent flow rate to Desolvation Temp and Gas Flow Rate.
Role of Cone Gas in reducing cluster ions and Purge Gas for removing solvent vapour.
How restrictor affects source pressure and sensitivity, and adjustment precautions.
Typical and maximum source temperatures, APcI probe temperature settings.
Optimal voltage ranges for capillary and sample cone for electrospray.
Adjusting RF Lens 1/2 and Aperture voltages for sensitivity and fragmentation.
Optimizing mass resolution and ion energy for peak shape and resolution.
Adjustments for megaflow: drying gas, desolvation temp, source temp, probe position.
Steps to safely remove the probe from the source, including deselecting Operate.
Ensuring samples are dissolved, filtered, and using optimal concentrations.
Examples of reference samples like myoglobin and PEG for calibration.
List of common sample types analyzed in positive ion mode.
List of common sample types analyzed in negative ion mode.
Interfacing 1mm columns, using syringe pumps, and solvent mixing.
Interfacing 2.1mm/4.6mm columns, UV detector use, and solvent considerations.
Increasing sensitivity via LC (columns, trace enrichment) and MS (narrow scan, SIR).
Performing ESI at 5-1000 nl/min using glass capillary or Nano-LC options.
Description of nanoflow end flange, manipulator, stage, and rotation procedure.
Steps for removing probe and glass tube, warning about exposed heater.
Unscrewing pillars, fitting cone gas nozzle, connecting gas outlet.
Fitting O rings, perspex cover, and nanoflow end flange.
Attaching brackets, inserting light guide, and setting light source brightness.
Blocking gas outlets and connecting Capillary/Corona and ESI/APcI cables.
Controlling magnification with zoom lens and fine focus with objective lens.
Adjusting microscope focus by rotating its top.
Handling fragile capillaries, removing unions, passing nut/elastomer/union.
Tightening nut for capillary protrusion, loading sample, setting Capillary voltage, turning on nitrogen.
Restarting spray by adjusting manipulator to touch sample cone and shear glass hair.
Using back pressure to force liquid from capillary if spray stops.
Cutting tubing, setting ferrule position, threading fused silica through fitting.
Ensuring silica flush, tightening nut, placing O ring, threading silica through chamber.
Connecting capillary to syringe pump for flow check, cutting fused silica, attaching tubing.
Setting liquid flow, gas pressure, viewing spray, adjusting stage and capillary voltage.
Rotating stage outwards, removing/replacing protective cover and holder.
Connecting sprayer to HPLC system, detailing injection valve plumbing (P, C, S, W).
APCI for singly-charged molecules, suitability for LC-MS with high organic/aqueous phases.
Description of Z-spray source with corona discharge pin and heated nebuliser probe.
Importance of desolvation gas, background spectrum dependence on Cone setting.
Source assembly, nitrogen supply connection, and baffle fitting for APcI.
Ensuring probe heater is off, connecting PTFE tube to Nebuliser, checking gas flow.
Checking liquid jet flow, LC pump back pressure, nitrogen pressure, and aerosol formation.
Setting Source/Probe Temps, Corona, Cone, Desolvation Gas, Nebuliser Gas.
Adjusting Mass/Span, Gain, Probe position (in/out, sideways) for optimal ion beam.
Checking for a stable beam of solvent ions and referring to Sample Analysis hints.
Warnings about source enclosure temperature and switching off liquid flow before probe removal.
Typical parameters for qualitative analysis of mixtures.
Optimizing APcI conditions for quantitative MRM analysis using standard solutions.
Adjusting Corona current for optimal sensitivity based on compound polarity.
Adjusting probe position and APcI Probe Temp for maximum sensitivity.
Desolvation gas effect on chemical background noise levels, checked using MRM mode.
Turning off LC flow, setting probe temp, deselecting Operate, turning off nitrogen.
Warnings about burns risk and shortening probe heater life if removed when hot.
Importance of cleanliness, preparing workspace, handling components carefully.
Ensuring fans are unobstructed and checking/replacing fan filters.
How analyser pressure affects performance (resolution, noise) and LED indications.
Points to note before suspecting a leak: turbopump operation, rotary pump maintenance, over-temperature.
Basic points for locating leaks: suspect disturbed components, check flanges and O rings.
Pirani gauge requires no routine maintenance; Magnetron gauge cleaning refers to Edwards literature.
Purging oil of contaminants and returning oil mist from filter via gas ballasting.
Recommended continuous gas ballast for E1M18, manual operation for E2M28.
Cautions regarding frequent ballasting and venting with E2M28 manual gas valve open.
Maintenance of oil mist filter elements: odour and mist elements.
Replacing sorbent in foreline trap based on discoloration or schedule.
Checking oil level weekly, replacing oil based on color or intervals.
Steps for changing oil: gas ballasting, venting, draining, flushing, refilling.
Three basic parts: probe adjustment flange, glass tube, source flange assembly.
Removing probe flange and glass tube for access to cone, nozzle, pin, baffle, nanoflow interface.
Cleaning cone, nozzle, ion block, and hexapole lens, with warnings on solvents.
Steps for cleaning: launching tune page, switching off pumps/flow, setting temps, disconnecting gas.
Warnings about hot components, probe heater life, and using ultrasonic bath.
Disconnecting electrical connections and removing probe/flange/tube.
Rotating isolation valve to its fully anticlockwise position.
Disconnecting cone gas inlet line and using extraction tool.
Removing sample cone, gasket, and nozzle, wiping with swabs or ultrasonic bath.
Drying cone/nozzle with nitrogen, removing/cleaning/replacing baffle and liner.
Refitting sample cone/nozzle, noting isolation valve position.
Venting instrument, removing source enclosure, cone, nozzle as described previously.
Removing screws and washers to remove the cover plate from the ion block.
Warning that heater supply remains live until system is fully vented.
Removing screws from heater connections, straightening leads, unhooking thermocouple.
Removing screws securing ion block, withdrawing block, checking O rings.
Unscrewing restrictor bush, collecting washer, wiping restrictor end.
Immersing ion block in ultrasonic bath with solvents and drying components.
Removing ion block, screws for support, withdrawing ion tunnel carefully.
Ensuring O ring location and caution about scratching pumping block internal bore.
Immersing assembly in solvent for sonication and drying with nitrogen.
Removing differential aperture plate, cleaning with glass fibre pencil and ultrasonic bath.
Checking O rings, feeding hexapole transfer lens with springs.
Replacing ion block support, screws, restrictor, bush, washer, and ion block.
Inserting thermocouple, reconnecting heater leads, replacing cover plate.
Checking isolation valve closure and clicking Pump after instrument pumps down.
Procedure for cleaning or replacing the APcI corona discharge pin.
Unstable ion beam, peak broadening/tailing, high LC pump back pressure.
Clearing blockages in sample capillary or tubing, using formic acid.
Flushing capillary with solvent or reverse flushing to clear blockages.
Checking all gas connections for leaks using soap solution or leak searching agent.
Removing probe, LC line, nut, grub screw, cover, and probe tip.
Removing old capillary, remaking LC connection, sleeving new capillary.
Using GVF/16 ferrule and adapter nut to connect capillary to LC union.
Feeding capillary through probe, adjusting tip protrusion, replacing cover and tightening grub screw.
Probe tip contamination, peak broadening/tailing, low/high LC pump back pressure.
Removing deposits from probe heater with a brush soaked in methanol:water.
Loosening grub screws to remove probe tip assembly, disconnecting heater.
Fitting a new heater assembly and reconnecting the probe tip assembly.
Removing probe tip assembly, heater, end cover, retaining screws, and loosening filter.
Cutting new fused silica capillary and connecting it to the filter using ferrule and adapter nut.
Feeding capillary through probe, cutting at nebuliser, adjusting protrusion.
Removing polyamide coating, re-feeding capillary, tightening adapter nut, replacing cover.
Analyser quadrupoles should not require cleaning; pre-filter assembly cleaning infrequent.
Detector designed for trouble-free operation; seek assistance for issues.
Warning about high voltages present throughout the spectrometer.
Table listing fuse numbers, types, and reference numbers for various PCBs.
Checks for no beam: tuning, cables, Operate mode, source assembly, isolation valve, errors.
Checks for unsteady beam: gas/liquid flows, analyser pressure.
Causes of ripple: unstable power supplies, photomultiplier supply, vibration.
Method to distinguish noise types by setting ion energies negative.
Troubleshooting carry-over, LC contaminants, MRM transitions, solvent purity.
Adjusting ion counting threshold, comparing measurements, engineer investigation.
Causes of high back pressure (blockage) and methods to clear it.
Checking voltage readbacks and reassembly of source/hexapole transfer lens.
Further investigation requiring qualified service engineer personnel.
Using 600/1200 grade emery paper or lapping paper for metal components.
Solvents like IPA, Methanol, Acetone for cleaning, followed by blotting dry.
Gas ballast, checking oil level/colour, checking water chiller.
Changing oil mist filter elements, sorbent in foreline trap.
Changing oil mist filter mist element and changing rotary pump oil.
Format of calibration files (two columns: mass, intensity).
Contact information for Sigma chemical company for ordering samples.
Steps to read currently selected reference file into Notepad text editor.
Procedures for saving edited files, including new and overwrite options.
UBQ, HBA, SOD, HBB, MYO reference files for various analyses.
PEGH1000, PEGH2000, NAICS, NAIRB files for ES and APcI calibration.
Calculated m/z values for Horse Heart Myoglobin (myo.ref).
Calculated m/z values for Polyethylene Glycol (PEGH1000, PEGH2000).
Calculated m/z values for NAICS reference file.
Calculated m/z values for NAIRB reference file.
MYONEG, SUGNEG, NAINEG reference files for general, low mass, and ES calibration.
Calculated m/z values for MYONEG reference file.
Calculated m/z values for SUGNEG reference file.
Calculated m/z values for NAINEG reference file.
Preparation of PEG solutions with ammonium acetate for positive ion ES and APcI calibration.
Preparation of PEG solutions for extended mass range positive ion ES calibration.
Preparation of NaI/CsI solution for positive ion ES calibration, use of NAICS.REF.
Preparation of NaI/RbI solution for positive ion ES calibration, use of NAIRB.REF.
Suitability of solutions for negative ion calibration, use of NAINEG.REF.
