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Miltenyi Biotec MACSQuant Tyto - User Manual

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MACSQuant® Tyto® Cell Sorter
User manual
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Summary

Important Safety Information

Warnings and Precautions

General warnings and safety instructions for instrument operation and risk minimization.

Hazard Levels

Explanation of signal words used to warn about hazardous situations and property damage.

Safety Symbols

Description of symbols used to highlight conditions that could cause injury to personnel.

Safety Labels

Instructions on maintaining and inspecting safety labels and markings on the instrument.

General Safety Instructions

General guidelines for safe operation and troubleshooting of the instrument.

Electricity and Fire Hazards

Details potential risks from electrical devices, including shock, short circuits, and overheating.

Optical Radiation Hazards

Explains risks associated with laser and LED radiation and protective measures.

Chemical and Biological Hazards

Discusses risks from biological material, contamination, and chemical handling.

Servicing and Transportation

Guidelines for instrument servicing, repair, and safe transportation.

Disposal

Information on the disposal of electrical and electronic equipment (WEEE).

1 Introduction

1.1 The MACSQuant Tyto Cell Sorter

Overview of the MACSQuant® Tyto® Cell Sorter, its technology, and capabilities.

1.2 Intended Use

Defines the instrument's intended purpose and user restrictions.

2 Installation

2.1 Components Included in the Delivery

Lists all components provided with the MACSQuant® Tyto® Cell Sorter.

2.2 Consumables

Lists the consumables required for the operation of the instrument.

2.3 Unpack the MACSQuant Tyto Cell Sorter

Step-by-step guide for unpacking the instrument.

2.4 Install the MACSQuant Tyto Cell Sorter

Instructions for connecting the monitor and keyboard to the instrument.

3 Operate the Instrument

3.1 Switch On the Instrument

Procedure for powering on the MACSQuant® Tyto® Cell Sorter.

3.2 Switch Off the Instrument

Procedure for safely shutting down the instrument and software.

3.3 Scan a Cartridge

Steps for scanning the 2D code on the instrument cartridge.

3.4 Load a Cartridge

Instructions for correctly inserting the cartridge into the instrument.

3.5 Stop Sorting and Eject the Cartridge

How to stop the sorting process and remove the cartridge.

4 Maintenance

4.1 Decontamination of the MACSQuant Tyto Cell Sorter

Procedures for cleaning and decontaminating the instrument.

4.2 Replace Fuses

Guide on how to replace the instrument's fuses.

5 Troubleshooting

Cartridge Alignment Fails

Common issues and solutions when cartridge alignment fails.

Continuous Alignment Fails

Troubleshooting steps for continuous alignment errors.

Cartridge Cannot Be Pressurized

Possible causes and fixes for a cartridge failing to pressurize.

Valve Is Not Moving

Solutions for issues where the instrument's valve is not functioning.

Sort Channel Is Clogged

Troubleshooting steps for a clogged sort channel in the cartridge.

The Input Filter Is Clogged

How to address issues with a clogged input filter affecting sort rates.

False Negative Fluorescent Population

Diagnosing and resolving false negative populations in the analysis.

Low Yield

Reasons and solutions for low cell yield during sorting.

Low Purity of Target Cells

Troubleshooting steps for achieving higher purity of sorted target cells.

6 Technical Data and Specifications

Technical Data

Detailed technical specifications including dimensions, power, and ports.

Optics

Optical parameters of the MACSQuant Tyto Cell Sorter, including lasers and detectors.

Fluidics

Fluidic parameters such as sample volume, purity, and cell flow rate.

Data Management

Specifications for data management, measurement parameters, and signal processing.

Technical Data for External Monitors

Required technical specifications and certification for external monitors.

EC; EU Declaration of Conformity

Declaration of conformity for the laboratory equipment to EU directives and standards.

7 Technical Support

7.1 MACSQuant Live Support

Information on accessing real-time diagnostic service and remote assistance.

7.2 Contact

Contact details for technical support at Miltenyi Biotec headquarters.

8 Limited Warranty

Miltenyi Biotec MACSQuant Tyto Specifications

General IconGeneral
TypeCell Sorter
Number of Lasers3
Laser Wavelengths405 nm, 488 nm, 638 nm
Number of Detectors8
Fluorescence Detection Channels8
Purity>98%
Sample Volume Range100 µL to 5 mL
Cell Sorter TypeClosed system
Detection TechnologyFluorescence
Recovery>90%
Data Acquisition RateUp to 30, 000 events/second
Laser ConfigurationFixed
SoftwareMACSQuantify
SterilityClosed, sterile system
Dimensions (W x D x H)60 cm x 60 cm x 75 cm
Weight80 kg
TechnologyMicrochip-based cell sorting

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