Introduction
The Minimate
™
tangential flow filtration (TFF) capsule is the
central part of a system for performing concentration or
diafiltration on solutions of biomolecules. It is available with
low protein binding Omega
™
ultrafiltration membranes in
several different molecular weight cutoffs. The membranes
are encased in a polypropylene housing with luer connectors
on the feed, retentate, and filtrate ports for easy connection
to a pump and accessories.
• For Laboratory and process development applications
• Ideal for evaluation, process development, process
optimization, and validation studies
• Scaleable to LV Centramate
™
and Centramate
™
systems
• Easily connected up to Minim
™
System or peristaltic
pumps
• Integrity tested and testable by user
• All plastic construction
Applications
Tangential flow filtration is a rapid and efficient method for
the concentration or diafiltration of biomolecules. It can be
used in a wide range of applications.
• Concentrate and desalt proteins, peptides, or nucleic
acids (DNA, RNA, oligonucleotides)
• Recover antibodies or recombinant proteins from clarified
cell culture media
• Process metal sensitive enzymes and molecules
• Separate (fractionate) large from small biomolecules
• Recover or remove viruses from solutions
• Prepare samples prior to column chromatography
• Concentrate samples after gel filtration
• Replace dialysis applications
• Depyrogenate water, buffers, and media solutions
Product Information
The Minimate TFF Capsule comes with threaded luer plugs
in each of the 4 ports. A fitting package with the following
components is included.
Fittings Package
Part No. 88216 includes:
A Tubing, 3.2 mm (1/8") i.d., 120cm (4 ft)
B Male luer-to-hose-barb fittings, 3.2 mm (1/8"), 2
C Female luer-to-hose-barb fittings, 3.2 mm (1/8"), 2
D Tubing clamps, 4
E Retentate screw clamp, 1
F Adhesive Strip (hook and loop), 1
Instructions For Use
NOTE: Read about the capsules operating limits in the
specification section of Appendix B of this manual.
Required Equipment
• Pump
A peristaltic pump or equivalent with variable speed
control capable of delivering a constant flow rate from 10
to 100 mL/min/capsule at pressures up to at least 2 bar
(30 psi). The peristaltic pump head should be sized to
accept 1.6 mm (1/16 in.) or 3.2 mm (1/8 in.) i.d. tubing.
The Minimate fitting kit includes 3.2 mm (1/8 in.) i.d. tubing.
• Feed reservoir
Appropriately sized for the volume of sample or flushing
solutions, e.g. 100 mL or 500 mL. Using a reservoir where
liquid is drawn from the bottom allows the sample to be
concentrated to a smaller volume due to the reduced
hold-up volume
Recommended Equipment
• Pressure measuring device
Pressure gauge or transducer and display. At least one is
recommended on the feed port. Additionally, one on both
the retentate and filtrate ports may be added.
• Magnetic stir plate and stir bar
Provide adequate mixing in the reservoir
B
A
E
D
F
C
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