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General| Brand | Sartorius Stedim Biotech |
|---|---|
| Model | Sartobind Q Series |
| Category | Laboratory Equipment |
| Language | English |
Provides an overview of the Sartobind capsules and their applications.
Process to remove air from the unit completely before use, crucial for performance.
Procedure for cleaning the modules in place after unpacking using specific solutions.
Instructions for autoclaving the capsules at 121°C for 30 minutes for sterilization.
Table showing recommended flow rates for different capsule sizes to ensure optimal performance.
Guidelines for selecting equilibration buffer concentration and properties for protein precipitation prevention.
How pH affects binding to cation (Sartobind S) and anion (Sartobind Q) exchangers.
Using Sartobind Q for contaminant removal from products like monoclonal antibodies.
Adjusting sample conditions and prefiltering for optimal performance and to prevent blockage.
Washing procedure after sample loading in bind & elute mode using equilibration buffer.
Using salt concentration buffers to elute the target protein from the membrane.
Procedure for draining the capsule using air or nitrogen pressure to remove residual liquid.
Steps for regeneration and long-term storage of the capsules to maintain performance.
Information on the chemical stability of the capsules against common chromatography buffers.
Operating the Sartobind nano version with peristaltic pumps and LC systems.
Guidelines for scaling up purification processes from nano to larger capsules based on experiments.
Method for testing Sartobind Q membrane discs using BSA to determine binding capacity.
Method for testing Sartobind S membrane discs using lysozyme to determine binding capacity.
How to install capsules correctly for integrity testing with Sartocheck instruments.
Steps for performing the diffusion test to ensure capsule integrity.
List of available Sartobind capsules with their order numbers, descriptions, and quantities.
List of available accessories such as holders and trolleys with their order numbers.
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