INSTRUCTIONS MANUAL CODE 80158.02 REV 0 02/10/09 (It can be modified without notice) Page: 25
J.P. SELECTA s.a. Ctra. NII Km 585.1 Abrera 08630 (Barcelona) España Tel (34) 93 770 08 77 Fax (34) 93 770 23 62
e-mail: selecta@jpselecta.es - http://www.jpselecta.es
Material needed (for 6 samples):
•6 glass funnels Ø100mm
•6 filter papersp Ø100 normal, (Not slow ).
• 6 digestion tubes ( SELECTA Code 4042300).
•Digestion Bloc + RAT + Fume removal. ( SELECTA Codes 4000507 + 4000051 + 4005072 )
•6 erlenmeyer 500ml.
•Bath in order to hot the water at 60ºC
Reagents:
•1L. of HCl 4N ( 350ml de HCl 35% and complete until 1L. with distillated water ).
•Granules of purmice stone.
Procedure:
•Number the digestion tubes.
•Weigh around 3gr. of sample and to put it into the digestion tube.
•Add 150 ml of HCl 4N in each tube.
•Add a spoonful of granules of purmice stone.
•Put this into the bloc and to digest at 50ºC - 200ºC during 1 hour (see page 12 table). It has to boil
slowly.See the below photo.
•Retire the digestion Bloc and let cool down.
•Prepare the Erlenmeyer with the funnels and the filter paper. See the below photo.
•Number the erlenmeyer.
•Empty the content of the sample tube into the funnel with filter paper.
•Clean the tube with hot water ( ±60ºC ) y and empty into the funnel. To repeat it until water that goes out of
the tube is transparent.
•Put hot water ( 60ºC ) on the funnel.
•Empty the erlenmeyers.
•Repeat the operation until the water of erlenmeyer be transparent (at minimum 10 times).
•Let dry the paper filter.
•You will observe that keeps the fat of the sample.
•Proceed to the extraction with the det-gras equipment. Let dry 2-3h, and to extract in two phases: Boiling,
rinsing, let dry 30 minutesextraer en dos fases: Boiling, rinsing, dry 30 min
DG-04 PREVIOUS HYDROLISIS FOR THE FAT DETERMINATION: Cheese, egg and meat.
CET ESSAI DEMANDE LA MANIPULATION DE SUBSTANCES DANGEREUSES
MANIPULATION OF CORROSIVE PRODUCTS IS REQUIRED
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