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Spectro-UV SPECTROLINKER XL-1000 User Manual

Spectro-UV SPECTROLINKER XL-1000
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OPERATOR’S MANUAL
Microprocessor-Controlled
UV Crosslinkers
9/20 AM05048-4
Printed in U.S.A.
XL-1000 and XL-1500
TM
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Spectro-UV SPECTROLINKER XL-1000 Specifications

General IconGeneral
BrandSpectro-UV
ModelSPECTROLINKER XL-1000
CategoryComputer Hardware
LanguageEnglish

Summary

Unpacking and Inspection

Specifications

Control Panel Display Description

Operating Modes

Energy Mode

Sets energy dose using the number pad, compensated by UV integrator for automatic adjustment.

Optimal Crosslink Mode

Pre-programmed 120 mJ/cm² dosage for optimal membrane binding, with number keys deactivated.

Intensity Mode

Measures UV irradiance; displays 'BULB' if below 1,500 μW/cm², indicating tube replacement.

Time Mode

Sets exposure time up to 9,999 seconds for operations, with SECONDS LED indicating mode.

Applications

Crosslinking of DNA/RNA

Binds nucleic acids to membranes after blotting; 120 mJ/cm² optimal dosage for attachment.

Nicking DNA

Used to nick ethidium-bromide-stained DNA in agarose gels in place of a depurination wash.

Gene Mapping

Creates thymine dimers prior to restriction enzyme treatment for gene mapping.

Cleaning Procedure

Troubleshooting

Tube Replacement

Maintenance

Electrical Specifications

Environmental Conditions

Warranty

Ordering Information

References

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