Spectro-UV SPECTROLINKER XL-1500 User Manual

1
OPERATOR’S MANUAL
Microprocessor-Controlled
UV Crosslinkers
9/20 AM05048-4
Printed in U.S.A.
XL-1000 and XL-1500
TM
™

Spectro-UV SPECTROLINKER XL-1500 Specifications

General

Brand	Spectro-UV
Model	SPECTROLINKER XL-1500
Category	Computer Hardware
Language	English

Summary

Unpacking and Inspection

Specifications

Control Panel Display Description

Useful Conversions

Operating Modes

Energy Mode

Operates in ENERGY mode with microjoules/area displayed. Dosage up to 0.9999 J/cm².

Optimal Crosslink Mode

Pre-programmed for 120 mJ/cm² (120,000 µJ/cm²). Number keys deactivated.

Intensity Mode

Displays irradiance at chamber center. Unit stays on until RESET. μW/cm² LED indicates mode.

Time Mode

Operates in TIME mode with seconds displayed. Exposure up to 9,999 seconds. SECONDS LED lights.

Applications

Crosslinking of DNA/RNA

Binds nucleic acids to membranes post-blotting. Optimal dosage 120 mJ/cm² for attachment.

Nicking DNA

Nicks ethidium-bromide-stained DNA in agarose gels, replacing depurination wash.

Gene Mapping

Creates thymine dimers before enzyme treatment to inhibit cleavage.

Testing and Screening RecA

UV Sterilization

Eliminating PCR Contaminants

Miscellaneous Uses

Cleaning Procedure

Troubleshooting

Tube Replacement

Maintenance

Electrical Specifications

Environmental Conditions

Warranty

Ordering Information

References