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Takara Bio SMARTer ICELL8 cx - User Manual
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Takara Bio USA
, Inc.
1290 Terr
a Bella Ave
nue, Mountain Vie
w, CA
94
043, USA
U.S. Technical S
upport:
techUS@
takarabio.com
United States/
Canada
800.662.2566
Asia Pacific
+1.650.919.730
0
Europe
+33.(0)1.3904.
6880
Japan
+81.(0)77.565.
6999
Page 1 of
35
Takara Bio USA,
Inc.
SMARTer™ ICELL8
®
cx Single-
Cell System
User Manual
Cat. No
s.
640188,
640189,
640187, 640002, 6
40198
(061918)
2
Table of Contents
Main Page
Table of Contents
2
Introduction
5
Thank You for Your Order
5
About this Manual
5
Technical Support
5
Table I. User Manual Symbols and Conventions
5
ICELL8 CX Safety Information
6
System Description: Component Overview
9
Table of Figures Figure 1. the ICELL8 CX Instrument
10
System Description: Instrument Overview
10
Figure 2. Water Bottle Seated on the Electronic Scale
11
Figure 3. Humidifier Unit
11
Figure 4. Syringe Pumps Unit
12
Figure 5. ICELL8 CX Stage Module
12
ICELL8 CX Specifications and Lab Requirements
13
Table II. ICELL8 CX Specifications and Lab Requirements
13
Setup and Installation
14
Required Equipment and Supplies from Your Lab or Other Suppliers
14
Table III. ICELL8 CX Installation Kit Components
14
Required Materials from TBUSA
15
Table IV. Materials from TBUSA Required for All Applications
15
Figure 6. ICELL8 CX Workflow
15
System Description: Smartchip Technology
15
Table V. Quick Reference Guide
16
Protocol: Quick Reference Guide
16
Protocol: Preparing the ICELL8 CX System
17
Powering on the System
17
Checking System Containers
17
Figure 7. Example of Dewpoint Parameter Display
17
Running the Daily Warmup
18
Figure 8. Wash Container
18
Figure 9. Startup Tab
18
Figure 10. Daily Checklist
18
Running the Tip Clean Procedure
19
Figure 11. the [Tip Clean] Button in the Manual Control Section
19
Protocol: Preparing the Source Plates
20
VIII. Protocol: Dispensing Samples into the Chip
20
Selecting the Smarter ICELL8 CX Application
20
Figure 12. Source Plate Map
20
Figure 13. Single Cell / TCR Tab
20
Placing the Chip in the ICELL8 CX Unit
21
Placing the Sample Source Plate in the ICELL8 CX Unit
21
Figure 14. Placing the Chip on the Dispensing Platform
21
Figure 15. Empty Plate Nest for the Sample Source Plate
21
Dispensing the Sample Cells, Fiducial, and Controls into the Chip
22
Figure 16. View of the ICELL8 CX with the Front Door Closed and Open
22
Figure 17. Select the 3'DE / TCR Tab
22
Figure 18. Vacuum Status Confirmation
23
Figure 19. Confirm Plate and Chip Loading
23
Figure 20. Confirm Seal Removal
23
Figure 21. Live Image of Chip Scanning
24
Figure 22. Input Chip ID
24
Figure 23. Vacuum Status after Dispense
24
Imaging the Chip
25
Figure 24. Initiate Chip Scanning
25
Figure 25. Remove the Chip Seal
25
Figure 26. Enter the Chip ID after Autofocus and Barcode Scanning Steps
26
Figure 27. Select Analysis Setting and Barcode Gal Files
26
Figure 28. Select Path and File Name for Saving Data
27
Figure 29. Naming and Saving Results File
27
Blotting and Sealing the Chip
28
RT Dispense
29
Figure 30. Cell / RT Dispense and Scanning
29
Figure 31. RT-PCR MIX Source Plate Map
29
Figure 32. Initiate RT Dispense
29
Figure 33. Loading Filter Files
30
Figure 34. Confirm Removal of Sealing Film
31
Figure 35. Live Image of Chip Scanning
31
Running Your PCR on a Thermal Cycler
32
Cleaning the ICELL8 CX System
32
Figure 36. Enter Chip ID
32
Figure 37. Confirm that Chip ID Matches Filter File Name
32
Maintenance
33
Daily Maintenance
33
Complete Shutdown Procedure
33
Common Replacement Parts
33
Bi-Annual Preventative Maintenance
33
IX. Maintenance
33
Troubleshooting
34
Table VI. Problem: Drop Dispersion, or Improper Dispensing
34
Table VIII. Problem: Dispensing Head Does Not Home
34
Table IX. Problem: Low or Partial Dispenses
34
Technical Support
35
Table X. Problem: Apparent Low Sample Concentration
35
Table XI. Problem: System Stalls Because the Syringe Does Not Move
35
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Takara Bio SMARTer ICELL8 cx Specifications
General
Brand
Takara Bio
Model
SMARTer ICELL8 cx
Category
Laboratory Equipment
Language
English
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