OperatingtheThermoScientificSorvallLegendMicro21RCentrifuge
Switchingonthecentrifuge
Setthemainpowerswitchoftheinstrument.Thedisplayshowsthattheinstrumentisgoingthroughan
internalcheckofitssoftware.Afterthischeckthedisplayswitchedintothecurrentvaluemode.The
speedandtheremainingruntimeshow0;therefrigerationunitdisplayshowsthecurrenttemperature
ofthesample.
Openingthelid
Themainpowermustbeontoopenthelid.Toopenthelidpressthe“open‐lid”button.
Closingthelid
Thecentrifugelidislockedbyslightlypressingdownthefrontpartofthelid.DONOTSLAMTHELID!
Fillingthecentrifugetubes
Thesmallerthecentrifugeimbalance,thebettertheseparation,becauseseparatezonesarenolonger
perturbedbyvibration.Itisthereforeimportanttobalancethecentrifugetubes
aswellaspossible.To
minimizeimbalanceyoushouldfillthetubesaseve nlyaspossible.Youcanachievethisbyeye,
however,youmustnonethelessensurethatoppositetubesarefilled tothesamelevel.
Placingthetubesintherotor
Therotormustbeloadedsymmetrically.Whenloadingtherotoronlypartially,youmustensurethat
oppositeboresalwaysreceivetubesofequalweight(whencentrifugingasinglesample,placea
centrifugetubee.g.filledwithwateropposite).Thefollowinggivesexamplesforproperloading: