Tissue-Tek Film User Manual

Tissue-Tek
®
Film
®
Automated Coverslipper
Ensure
Quick Reference Guide
The Tissue-Tek
®
Film
®
automated coverslipper is
designed to coverslip biological specimens that are
mounted on standard 25 x 75 mm (1.0 x 3.0 inch)
microscope slides. The use of a special, resin-coated,
film and xylene eliminates the need for coverglass and
mounting media. The instrument, as part of the
histopathology process, is intended to facilitate the in
vitro examination of human and animal tissue for
morphology changes by a pathologist.
This Quick Reference Guide contains information about
day-to-day use, maintenance and cleaning. For more
detailed information we refer to the most recent
Operating Manual of this instrument.
All users should be trained for the use of the Sakura
instruments. The Sakura User Training is a training to
ensure that new and existing users of a Sakura
instrument have the knowledge and skills to operate and
maintain the instrument in a proper way.
Daily operations
To operate the instrument switch on the “POWER”
button located near the control panel.
By turning on the power there will be a pre-operational
check.
Check the volume of xylene bottle and empty the waste
bottle if necessary. When air bubbles are in the line
press “PRIME” to remove them.
Ensure the Film is placed in the instrument as indicated
in the image on the right. Follow the numbered
positioning spools and rollers until it has reached
number eight. Now Press “FILM” to feed the film
automatically through the system until you can grab it at
the end.
When the Film is properly fed press the cutter blade
down to signal the system it is ready for use. Remove
any access Film cut off in this way.
Version : 2019-1

Tissue-Tek Film Specifications

General

Brand	Tissue-Tek
Model	Film
Category	Laboratory Equipment
Language	English

Summary

Daily Operations and Initial Setup

Instrument Power On and Pre-Operation Check

Procedure for switching on the instrument and understanding the pre-operational check sequence.

Xylene and Waste Bottle Management

Checking Xylene volume and emptying the waste bottle as part of daily checks.

Automated Film Loading

Step-by-step guide to correctly load the Film into the instrument using positioning spools.

Cutter Blade Preparation

Action to prepare the cutter blade after successful film feeding for use.

Loading Station Initialization and Basket Handling

Initializing the loading area and procedures for manual basket loading and slide placement.

Slide Drying and Post-Operation Film Handling

Drying slides in the carousel and storing the Film correctly after daily operations.

Film Removal and Storage

Coverslipping Film Removal Procedure

Detailed steps to remove the coverslipping Film from slides using acetone and ethanol.

Storage Guidelines for Unused Film

Recommendations for storing unused Film, including environmental conditions and acclimation.

Instrument Maintenance

Exterior and Interior Surface Maintenance

Cleaning procedures for the instrument's exterior and internal components.

Waste and Xylene Bottle Maintenance

Procedures for emptying the waste bottle and refilling the Xylene bottle.

Component Maintenance and Replacement

Maintenance for slide baskets, rollers, carbon filters, and film waste tray.

Cutter Blade and Preventative Maintenance

Guidelines for cutter blade replacement and scheduling yearly preventative maintenance.