The UVITEC UVIdoc HD6 is a high-end ultra-sensitive scientific camera platform designed for the detection of protein or DNA samples. It offers superior sensitivity, resolution, and dynamics, providing optimal performance across a large array of applications. The system is designed to capture fluorescence or colorimetric gel images and utilizes UVITEC-1D software for image capture and optimization for selected applications. It is capable of capturing high-resolution digital images of protein and DNA bands in gels obtained by electrophoresis separation methods, as well as images of fluorescent and colorimetric samples, depending on the system configuration. The instrument is suitable for research purposes in academia and the life sciences industry, and for diagnostic purposes.
Function Description:
The UVIdoc HD6 imaging system is powered by mains voltage and equipped with a UV light source, used to capture images of samples that may be hazardous. The system provides advanced imaging capabilities for various applications, including 1D DNA gel, 1D protein colorimetric samples, multiplexing, stain-free gel, and blot imaging. It offers exquisite precision and resolution, with reliable results for both quantification and documentation. The advanced imaging electronics, high-quality optics, and advanced software deliver outstanding performance. The UVIdoc HD6 system separates emitted light from the excitation light to obtain an optimum sample image. The system can accommodate several excitation and emission channels in the visible and UV area.
Important Technical Specifications:
- Power Supply:
- Current (A): 1A/0.5A
- Fuse FST (A): 2A
- Voltage (V): 100/230V~ (5%)
- Frequency (Hz): 50/60Hz
- Power: 85 watts
- Fuses:
- Type: FST
- Time-lag: T
- Dimensions: Ø5 x 20
- Rating: 2A
- Climatic Conditions:
- Altitude: 2000 meters
- Operating humidity: 20% to 70% (no condensation allowed)
- Operating temperature: The maximum ambient temperature should be 25°C.
- Image Resolution: 16-bit imaging, capable of 65,535 gray levels.
- Connectivity: USB ports (USB3 for camera, 4 alternative USB ports), LAN network port.
- Weight: 30kg (imager only).
- Dimensions: LCD panel operation free space required: 50 cm. Right, left, rear, and top minimum free space required: 10 cm.
Usage Features:
The UVIdoc HD6 system is designed for ease of use with a quick start user guide.
- System Installation: The system is unpacked, and hardware is installed, including connecting the power cable and USB cables. The imager weighs 30kg and requires two people for placement on a stable laboratory bench or table capable of supporting 100 kg.
- Environmental Conditions: The instrument should be placed on a level-working surface, away from water, solvents, or corrosive materials. It must not be installed near a sink or in humid (above 70% RH) or dusty environments. Adequate cooling requires at least 40 cm of free space in front of the instrument and at least 10 cm on all other sides. The system should be placed away from interfering electrical signals and magnetic fields.
- Operating Instruction:
- Darkroom Usage: Warnings are provided for safe operation within the darkroom, including switching off the transilluminator when gel is not present on the UV filter to prevent damage, waiting at least 20 seconds in "High" position before reducing intensity to "Low," and changing all 6 tubes simultaneously if one or several tubes are off or used for better homogeneity.
- Imager Front Side - Door Open: The system features intelligent darkroom technology with software control of lighting, automatic white light intensity adjustment, and precalibrated focus. The slide-out transilluminator and UV security switch (9) and on/off button (8) are accessible when the door is open.
- Filter Wheel: A 3-position filter wheel is equipped with the F590 M58 interferential filter for Ethidium bromide application. The F590 Ethidium bromide filter is attached to position 1. Filters can be selected by turning the black handle.
- Software Operation: The system starts by pressing the power button on the left side of the imager. The software guides users through steps: Start the UVIdoc HD6 system & software, Position your sample, Capture an image, Visualize your image, and Save and print.
- Image Capture Workflow: The basic steps include choosing the application type (e.g., UV-Gel), acquiring the image, and saving or printing the image. Pre-defined image acquisition parameters facilitate system use.
- Exposure Modes: The system offers "live mode" for direct visualization, "auto mode" for automatic optimum exposure time calculation, and "start mode" for user-specified exposure time.
- Image Display: The display option menu enhances image display by modifying the image grayscale selection. The software handles two images: a "memory" image (16-bit format, 65,536 grey levels) and a "displayed image" (256 grey levels). The software allows selecting grey level ranges to translate for display image calculation, offering presets from 20%, 40%, 60%, or 100% of the image depth.
- Application Protocol: The software runs with pre-defined image acquisition parameters. The factory settings include several pre-defined Application protocols, and users can create their own.
- Advanced Exposure: The Advanced Exposure folder contains exposure pre-defined set-ups, including zoom and big size options, lighting (fluorescence mode only), and extended resolution.
- Fast Mode / Standard Mode: The fast mode provides lower image resolution to speed up the imaging process.
- Auto-exposure Set-up: The software analyzes a test image to calculate an ideal exposure time.
- Manual Exposure Set-up: Defines the default imaging exposure time, with scales for short times (40 milliseconds to 4 seconds) and long times (4 seconds to 1 minute).
- Preview - Live Preview Pre-defined Parameters: The Preview folder contains live review pre-defined set-ups, including extended resolution and lens prefixed zoom position table.
- Display - Image Display Pre-defined Parameters: The Display folder contains image display pre-defined set-ups, including select display mode, set the gamma value, display the saturation, and select the color palette.
- Printing - Image Printing Pre-defined Parameters: The Printing folder contains image printing pre-defined set-ups, including print with image name, print with date and time, print out text color, and free text print out.
- General - Image File Pre-defined Parameters: The General folder contains image file pre-defined set-ups, including user's name, protocol's avatar, default file name/image name prefix, file format, preferred image saving directory, and autosave options.
- System Settings: Settings feature allows adjustment of system settings such as filter controls or lens calibration. It displays information like imaging system version, camera serial number, USB status, installed filters, Epi-Light module, transillumination, and tray calibration.
- Windows Parameters: Users can update or reset, lock or unlock the system, and set its network address. The UVIdoc HD6 system is write-protected to prevent installation of unauthorized programs or files.
- Network Parameters: The UVIdoc can be connected to a local area network (LAN) via TCP/IP. IP addresses need to be provided for proper connection.
- Firmware Update: Firmware can be updated by placing files on a USB stick and running the setup.exe file.
Maintenance Features:
- Warranty: The UVIdoc imaging system is warranted against faulty construction or defective material for a period of two years from the invoicing date. The warranty is voided if the instrument is modified or altered in any way.
- Cleaning: To clean the surface of the filter, use mild solvent or warm water. Dry with a soft cloth. The filter is porous, so try to keep it dry. Do not use abrasive cleaning materials or excessive amounts of liquids.
- Spare Parts: Information on spare parts (fuses, tubes, filters) is provided, along with their respective part numbers.
- Safety: The user manual contains safety notices (WARNING, UV WARNING, NOTICE) concerning the safe use of the product. It emphasizes precautions against physical damage, exposure to irradiated light or electric shock, injury from touching an operating part, dangerous atmosphere, fire or shock hazards, obstruction of ventilation, use of unauthorized accessories, handling hazardous chemicals, and improper lifting.
- Electrical Safety: Connect the UVIdoc imaging system to an appropriate AC voltage outlet that is properly grounded and protected by a circuit breaker. Do not damage the power supply cord. Do not block access to the power switch and power cord. The UVIdoc imaging system has serviceable fuses located on the rear panel.
- Decontamination, Collection, and Elimination of Waste: The buyer ensures and finances the decontamination, collection, and disposal of waste electrical and electronic equipment (WEEE) under the conditions provided in the Articles 21 and 22 of the Decree No. 2005-829 dated 20 July 2005. For tubes recycling, contact Recylum (www.recylum.com).
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