VWR avantor - Operating Instructions; Recommended Immersion Depths; Aspiration and Dispensing Instructions; Aspirating Liquid Procedure

12 – VWR STANDARD LINE PIPETTORS Instruction Manual – 13
7. Operating Instructions
Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid
sampling.
• Ensure smooth and slow operation of the pipettor.
• Immersion of the tip into the sample liquid should be
kept to a minimum depth, which should remain con-
stant during aspiration. The recommended immersion
depths are given in the table below.
Model Volume Range (μL) Immersion Depth (mm)
0.1-1 ≤1
1-100 2-3
101-1,000 2-4
5,000 3-6
10,000 5-7
• The pipettor should be held in a vertical position.
• The pipet tip should be changed whenever the vol-
ume setting is altered, and when a different liquid is
to be aspirated.
• The pipet tip should be changed if a droplet remains
on the end ofthe tip from the previous pipetting
operation.
• Each new pipet tip should be pre-rinsed with the
liquid to be pipetted.
• Liquid should never enter the pipettor shaft.
To prevent this:
– Press and release the pushbutton slowly and
smoothly.
– Never turn the pipettor upside down.
– Never lay the pipettor on its side when there is
liquid in the tip.
• Never force the volume setting beyond therecom-
mended limits.
• When pipetting liquids with a different temperature
from the ambient temperature, it is recommended to
pre-rinse the tip several times prior to use.
• Do not pipette liquids with temperatures above 70°C.
NOTE: When pipetting acids or corrosive solutions
which emit vapors, it is recommended to disassemble
the shaft and rinse the plunger and O-ring with distilled
water after finishing the pipetting operation.
8. Aspiration and Dispensing Instructions
1. 2. 3. 5.4. 6.
Aspirating Liquid
1. Press the pushbutton to the first stop point.
Holdingthe pipettor vertically, immerse the pipet tip
into the sample liquid to the recommended depth (for
recommended values see Section 7). Ifthepipet tip
is not immersed to the recommended depth or if the
pushbutton is rapidly released, air may enter the pipet
tip.
2. Release the pushbutton slowly and smoothly to aspi-
rate the sample. Wait one second and then withdraw
the pipet tip from the liquid.
CAUTION: Do not touch the used tip.
Dispensing Liquid
3. Place the end of the pipet tip against the inside wall
of the vessel at an angle of 10° to 40°. Press the push-
button smoothly to the first stop. Wait one second.
4. Press the pushbutton to the second stop to expel
any remaining liquid. While keeping the pushbutton
depressed, remove the pipettor from the vessel by
drawing the pipet tip against the inside surface of
thevessel.
5. Release the pushbutton to its starting position.
6. Eject the pipet tip by pressing the tip ejector
pushbutton.
Remember to change the pipet tip whenever
a different type of liquid is to be sampled.