Installation and Verification of Other Sources 4
To verify the operation of the G1971B APPI source
Agilent 6400 Series Triple Quad LC/MS System Installation Guide 81
To verify the operation of the G1971B APPI source
There is no sensitivity checkout with the G1971 APPI source on the Triple
Quadrupole LC/MS. To verify its proper operation, run a manual tune with the
G2432A APCI/APPI Calibration Solution.
Note that Auto Tune is not supported with this source.
1 Install the G2432A APCI/APPI Calibration Solution.
2 Check the tuning of the APPI source:
a In Data Acquisition program, set Context to Tune.
b Open the auto tune file that was generated with the electrospray or
multimode source.
c In the Manual Tune tab, mark the Calibrant on check box, and then
acquire the data.
d Verify that the masses in the MS1 and MS2 tabs are correct for the
loaded source before you manually tune the peak.
e Verify that you have sufficient abundance for the tune peaks, that the
tune peaks have peak widths of 0.7 ±0.05 amu, and that mass
assignments are within ±0.15 amu of the target mass assignments.
If needed, click Adjust Gain & Offset to establish the Mass Axis and
Peak Width values. Repeat these for both MS1 and MS2 tabs.
3 Save the tune file.
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