12
1 Introduction to the Autosampler
Sampling Sequence
Next, the needle is raised, and the vial is positioned below the needle. The
needle moves down into the vial, and the metering unit draws the sample into
the sample loop (Figure 4 on page 12).
Figure 4 Drawing the Sample
When the metering unit has drawn the required volume of sample into the
sample loop, the needle is raised, and the vial is replaced in the sample tray.
The needle is lowered into the needle seat, and the injection valve switches
back to the mainpass position, flushing the sample onto the column (Figure 5
on page 12).
Figure 5 Mainpass Position (Sample Injection)