ENGLISH
• T
he use of a guard column is recommended to protect your column and increase
its lifetime.
•
Columns should not be maintained at high pH or elevated temperature when not
in use.
•
Avoid use of this column outside recommended pH range for column phase (see
next page). Expect reduced lifetime when operating outside the recommended
pH and temperature ranges.
Tips for Getting the Best Chromatographic Results
•
Optimize your instrument by minimizing tubing lengths between components to
reduce extra column volume and band broadening. Use 0.12 mm id red or 0.075
mm id black tubing for Fast LC/high efficiency columns. Learn about capillary
options at www.agilent.com/chem/lccapillaries.
•
When using normal-phase columns, ensure that your LC instrument’s rotor seals,
pump seals, tubing, and all other components are compatible with normal-phase
solvents.
•
Make sure your data collection rate is optimized for your column. Use a higher
collection rate for Fast LC columns (
InfinityLab
Poroshell 120, RRHT and RRHD).
•
Use Agilent certified lamps in your LC instruments for best performance.
•
Use sample filtration or other sample prep as appropriate for your sample. Learn
more at www.agilent.com/chem/sampleprep
If you are using InfinityLab Poroshell 120 or ZORBAX RRHT or RRHD
columns, use a Fast Guard for UHPLC to protect your analytical column.
See www.agilent.com/chem/fastguards for more information.
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