Cleaning Your Column/Extending Column Life:
HILIC Columns
Samples should be filtered before injecting on the column. This is particularly
important for the ZORBAX RRHD HILIC Plus column, as the column inlet frit is
nominally 0.5 µm and samples should be filtered through a 0.2 µm sample filter.
If solvent flow appears to be restricted (unusually high column backpressure),
check first to see that solvent flow is unobstructed up to the column inlet. If the
restriction is prior to the column, replace the appropriate piece of tubing or filter
that is plugged. If the column is plugged, InfinityLab Poroshell 120 HILIC can
be backflushed with 20-30 column volumes of mobile phase. The RRHD HILIC
column cannot be backflushed. To remove strongly-retained materials from the
column, flush the column with a strong solvent which in HILIC mode, is water.
Storage Recommendations: HILIC Columns
Acetonitrile:water (95:5) is recommended as the long-term storage solvent for
HILIC columns. It may be necessary to flush the column with acetonitrile:water
(60:40) to remove strongly-retained compounds prior to switching to the storage
solvent. Before storing the column, tightly cap the end fittings with the end plugs
to prevent the packing from drying out. Columns may be safely stored for short
periods in most HILIC mobile phases. However, to protect equipment, it is best
to remove salts from the instrument and column by purging the column with the
same mobile phase without the buffer (for example, using 90:10 ACN:H
2
O to
remove a 90:10 ACN:0.01M formate buffered mobile phase). Re-equilibration is
faster with the original mobile phase when using this approach, but several (3 to
6) injections should be made to verify column equilibration.
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