Column Usage Tips
•
Modern columns are robust and are designed to operate for long periods under
normal chromatographic conditions. You can maximize column lifetime by running
it within specifications. Always review the specifications before putting in place a
final method.
•
While generally not harmful to the column, reverse flow should be avoided except
to attempt to clear a clogged frit (see “Installation” for exceptions).
•
Always use high purity reagents and chromatography grade solvents or better to
prepare your mobile phase.
•
Disassembling a column will degrade column performance.
•
New columns contain a mixture of organic solvents. See your QC Performance
Report for the solvent composition in your column. Initially, care should be taken
not to pass any mobile phase through the column that may cause a precipitate or
an emulsion to form.
•
Agilent normal-phase columns are compatible with water and all common
organic solvents.
Column Type Particle Size Pressure Limit
InfinityLab Poroshell 120 1.9 µm 1300 bar (19500 psi)
InfinityLab Poroshell 120 2.7 µm, 4 µm 600 bar (9000 psi)
ZORBAX Rapid Resolution
High Definition (RRHD)
1.8 µm 1300 bar (19500 psi)
ZORBAX Rapid Resolution
High Definition (RRHT)
1.8 µm 600 bar (9000 psi)
All other ZORBAX, Pursuit,
Polaris
3.0 µm, 3.5 µm, 5 µm,
10 µm
400 bar (6000 psi)
Rx, Metasil, Monochrom,
Microsorb
3.0 μm, 3.5 μm, 5 μm,
10 µm
400 bar (6000 psi)
Maximum Operating Pressures – Columns up to 9.4 mm id
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