Cleaning Your Column/Extending Column Life:
Normal-phase Columns
For normal phase, we recommend use of organic solvents. Use at least 20 column
volumes of each solvent, which is 50 mL if your column is the traditional analytical
4.6 x 250 mm. Try these solvents in order of increasing strength:
1. 50% methanol: 50% chloroform
2. 100% ethyl acetate
Cleaning a column used in normal-phase mode may depend on the sample type. If
these solvents do not work, contact Agilent so we can recommend a solvent that
may be more effective with your application and sample matrix.
Storage Recommendations: Normal-Phase Columns
Normal-phase columns can typically be stored in their own mobile phase for long
periods. Before storing the column, the end-fittings should be tightly capped with
end-plugs to prevent the packing from drying out.
WORKING WITH HILIC COLUMNS
HILIC is a method used for the separation of polar analytes inadequately retained
on typical reversed-phase columns. Agilent ZORBAX HILIC Plus and InfinityLab
Poroshell 120 HILIC columns are non-bonded silica columns, shipped in
acetonitrile:water or acetonitrile and ready to use for HILIC separations. ZORBAX
Carbohydrate columns are shipped in acetonitrile also ready for use in HILIC
mode. Like normal-phase columns, HILIC columns require more equilibration than
reversed-phase columns. Note that NH2, unbonded silica and CN columns can also
be used for HILIC applications. To change these columns to HILIC mode, flush first
with isopropyl alcohol.
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