Agilent Technologies ZORBAX XDB-CN User Manual

Recommended starting gradients
Stationary phase Usage notes
Most reversed-phase
columns
5% methanol or acetonitrile initially and 100%
methanol or acetonitrile as the nal solvent.
ZORBAX Eclipse PAH* 30 or 40% acetonitrile initially, to 100%
acetonitrile as nal solvent. It may be necessary
to cool column to 15 to 20 °C for improved
resolution.
ZORBAX Bonus-RP Lower concentration of organic mobile phase
modier is required for compound elution,
compared to traditional long-chain alkyl
stationary phases.
Large molecule
columns (for example,
Poroshell300 or
ZORBAX300SB
columns)
Separations of polypeptides, proteins, DNA
fragments, etc. often use triuoroacetic or
formic acid modier for pH control and/or as
an ion-pairing additive for desired retention
and selectivity. Organic buffers such as TRIS
are especially useful for intermediate pH
applications.
* Best column lifetime is achieved at <40 °C.

Agilent Technologies ZORBAX XDB-CN Specifications

General

Brand	Agilent Technologies
Model	ZORBAX XDB-CN
Category	Laboratory Equipment
Language	English