BENSON BV200 - Quick Start Guide; Samples; Calibration

BV200 Plasma Viscometer Mk2/3 Customer User
Manual
Issue 9
th
Sep 2020 - Rev 1.8 Page 47 of 53
7. Quick Start Guide
 Empty Waste, Fill Wash (2 litre distilled water and 1 barcoded Wash Additve) empty and rinse
Condensation bottle
 Check Regulator is set between -0.40 and -0.45 and Waste Guage at -0.70 minimum
 Run Daily, Weekly or Monthly Maintenance as required
 Place a CHECK in the first green holder followed by 10 samples and another CHECK
 Click START, more samples and CHECK calibrators may be added at any time
 Place a STOP FLAG at the end of the sample line
7.1. Samples
 Allow all samples to stabilise at room temperature before testing. Remember this will take longer if samples
have arrived by post or from a courier
 Ensure all samples to be tested are fully centrifuged (note – controls do not need to be centirfuged)
Do not store PV samples in a fridge. When samples cool some cryoproteins can precipitate out,
Centrifugation may then remove this protein content from the sample - leading to an erroneous clinical
plasma viscosity result.
 If a known High Risk sample is tested, follow laboratory procedure for working with high risk samples and
decontaminate the analyser using a STERILISER tube
7.2. Vacuum
 When Calibrating use the HIGH and run blind (turn the barcode away so it cannot be read by the barcode
reader), adjust the regulator so the runtime is 2.00 ± 0.05
 Vacuum on waste bottle vac gauge should be a minimum of; -0.70
 Make sure the cap on the waste bottle is fully tightened and the cap tubes are fully inserted
7.3. Probe
 The probe air breather holes must be clear (10mm from the aspiration hole at the tip)
 Light should be seen directly through the breather holes
 Ensure the probe nut fitted to the cassette is as tight as possible using the red probe nut spanner
 Ensure the probe tip is centred on the probe target
 Probes can be aligned in the viscometer and do not need to be returned. – see Help, section 5.2.3. All
broken probes parts should be returned without the cassette, for repair or replacement
7.4. Cassette
 The capillary tube connector in the back of the cassette should be secure. It should not be able to be
unscrewed easily. If you have a red coloured tube connector, this is permanently fitted so do not try to
remove it
 The thin plastic tube at the back of the cassette should be pushed fully into the tube connector, through the
inner rubber seal until it cannot be pushed any further
 Check the penetration of the capillary at the probe aspiration hole. Ensure the capillary end can still be
seen but is not out over halfway. A „short‟ capillary end is considered best
7.5. Calibration
 Become familiar with calibration High (2.00), Low (1.32), and Check (1.67) run times
 Calibrate with the calibrator caps OFF, note any large variations or swings in gradient and offset
 BV200 gradient will normally be between 6.0 and 8.0