EasyManua.ls Logo

Bio-Tek ELx800 - User Manual

Bio-Tek ELx800
214 pages
Print Icon
To Next Page IconTo Next Page
To Next Page IconTo Next Page
Loading...
ELx800™
Absorbance Microplate Reader
Operator’s Manual

Table of Contents

Questions and Answers

  • T
    Tiffany WangSep 3, 2025
    What to do if Bio-Tek ELx800 Y-axis (carrier left/right) motor did not find the home sensor?
    • Z
      zwagnerSep 4, 2025
      Turn off the instrument. Upon restarting the instrument, you should be able to use the keypad. Possible causes: defective or broken optical sensor, sensor not connected, or Y-axis movement is limited so that the optical sensor cannot be interrupted.
  • A
    Alexis EspinozaSep 18, 2025
    What to do if Bio-Tek Laboratory Equipment abort occurred?
    • A
      Andrew MillsSep 18, 2025
      Consult the error code list to see if the error is easily correctable.
  • Y
    ywatsonSep 16, 2025
    What to do if Bio-Tek Laboratory Equipment abort error occurred?
    • P
      pam05Sep 16, 2025
      Consult the error code list to see if the error is easily correctable. The user may have aborted read from KC4, or from another serial interface.
  • K
    Kathryn SmithSep 14, 2025
    What to do if Bio-Tek ELx800 Laboratory Equipment read time dark value is out of range?
    • C
      Christina IrwinSep 14, 2025
      Reboot the system and/or check environmental conditions. The measurement electronics background noise has changed since the last power-up self-check, or ambient light may have increased since power-up.
  • M
    Matthew McguireSep 12, 2025
    What to do if Bio-Tek Laboratory Equipment autocalibration data is missing?
    • N
      Natasha ButlerSep 12, 2025
      Perform autocalibration. This issue occurs when autocalibration was not performed following assay definition download.
  • M
    Mary SchneiderSep 12, 2025
    What to do if Bio-Tek Laboratory Equipment wavelength not found in table?
    • E
      Erik CarterSep 12, 2025
      Run a system test. Verify that wavelengths in the filter wheel match settings in the filter table. The assay wavelength does not match the filter table.
  • M
    Melinda MeyerSep 9, 2025
    What to do if Bio-Tek ELx800 Laboratory Equipment failed configuration checksum test?
    • T
      Taylor HernandezSep 9, 2025
      Recreate the assay definition using the correct version of assay definition software and re-download the software. The configuration data has changed and the checksum stored is no longer valid. The error is produced when outdated versions of assay definition software are used to create an assay configuration file, making the file incompatible with the operation code within the instrument’s memory.
  • S
    Sarah FisherSep 7, 2025
    What to do if Bio-Tek ELx800 filter is not installed?
    • A
      Amanda OconnorSep 7, 2025
      Turn off the instrument. Upon restarting the instrument, you should be able to use the keypad. This issue may occur if there is an empty filter location on the filter wheel (all filter locations must have either a filter or a filter blank, Bio-Tek PN 2872086), or if the entire filter wheel is not installed.
  • S
    samuel71Sep 6, 2025
    What to do if Bio-Tek ELx800 Laboratory Equipment X-axis (carrier in/out) motor did not find the opto (home) sensor?
    • J
      Jackson RamirezSep 6, 2025
      Turn off the instrument. Upon restarting the instrument, you should be able to use the keypad. Possible causes include a defective or broken optical sensor, a disconnected or damaged flex cable, or X-axis movement being limited so that the optical sensor cannot be interrupted.
  • D
    Dr. Antonio Gilbert MDAug 20, 2025
    What to do if Bio-Tek ELx800 Laboratory Equipment reader failed noise test?
    • H
      Hayley ReevesAug 21, 2025
      This failure indicates excessive variation in the dark current (background) noise levels of the measurement circuit. External signals might be penetrating the measurement chamber. Make sure that the bottom and top shrouds, and the filter wheel cover, are correctly installed. The cause may be a faulty motherboard (PN 7330400, 7330410, or 7330415) or daughter board (PN 7330404 or 7330414). The photodiode or power supply may be too close to the instrument.

Summary

Chapter 1: Introduction

Introducing the ELx800 Automated Microplate Reader

Describes the ELx800 reader's purpose and capabilities as a single-channel reader-assay system.

Hardware Features

Details the physical components and specifications of the ELx800 instrument.

Software Features

Outlines the functionalities and capabilities of the ELx800's embedded software.

Package Contents

Lists all items included in the ELx800 package upon purchase.

Optional Accessories

Lists available add-ons and upgrades to enhance the ELx800's functionality.

Standard Model Specifications

Specifies performance parameters for the standard ELx800 configuration.

Ultraviolet;UV Model Specifications

Details specifications specific to the UV-capable ELx800 model.

Narrow Beam;NB Model Specifications

Lists specifications for the ELx800 model with narrow beam capability.

Technical Support

Provides contact information and procedures for obtaining technical assistance.

Chapter 2: Installation

Unpacking and Inspecting the ELx800

Instructions for safely removing and checking the instrument and its accessories upon arrival.

Setting Up the ELx800

Guides on configuring the instrument's environment and connections.

Power-Up and System Test

Describes the initial power-on sequence and the automatic system self-test.

ELx800 Main Menu

Introduces the primary navigation interface of the ELx800.

Configuring Global Default Options

Explains how to set up general instrument preferences and settings.

Connecting a Printer to the ELx800

Provides steps for connecting a compatible printer to the reader.

Setting Up the Serial Port for Communications With Other Devices

Guides on configuring the serial port for data transfer and control.

Installing Additional Filters

Instructions for physically installing or changing optical filters in the instrument.

Repackaging and Shipping the ELx800

Detailed instructions for safely packing the instrument for transit.

Chapter 3: Operation

ELx800 Front Panel

Describes the physical interface and controls on the front of the ELx800.

Overview

General introduction to operating the ELx800 and its software.

Recommendations for Achieving Optimum Performance

Tips and guidelines for maximizing the accuracy and reliability of measurements.

System Startup

Procedures for powering on the ELx800 and initiating its self-test sequence.

Main Menu

Details the functions and navigation options available from the main operational screen.

Define

Guides on creating, modifying, and managing assay protocols and parameters.

Reading a Microplate

Step-by-step process for performing a measurement run with a microplate.

Printing Reports

Options and procedures for generating and printing assay results and data.

Using the Utility Options

Accessing and configuring instrument settings and preferences.

Chapter 4: Performance Verification and IQ;OQ;PQ Procedures

Recommendations for Achieving Optimum Performance

Guidelines to ensure accurate and reliable instrument operation.

Recommended Test Schedule

Defines intervals for routine performance testing of the instrument.

System Test

Verifies the instrument's internal components and light path functionality.

Checksum Test

Confirms the integrity of the instrument's software programming.

Absorbance Plate Test

Assesses mechanical alignment, accuracy, and linearity using a test plate.

Test Failures

Actions to take if the Absorbance Plate Test parameters report a "FAIL".

Liquid Testing

Procedures for verifying performance using liquid samples.

Chapter 5: Maintenance and Decontamination

Maintenance

General procedures for keeping the instrument in good working order.

Replacing the Bulb

Instructions for safely replacing the instrument's lamp.

Decontamination Procedure

Protocols for decontaminating the instrument, especially before shipping or servicing.

Chapter 6: Troubleshooting and Error Codes

Overview

Introduction to diagnosing and resolving instrument issues and error messages.

System Test Description

Explains the purpose and function of the ELx800's internal system test.

Glossary of Terms

Definitions of key terms used in troubleshooting and error code sections.

Error Codes

Lists and explains the various error codes displayed by the ELx800.

Appendix A: Controlling the Reader With KCjunior or KC4

Overview

Introduces using PC software for enhanced reader control and flexibility.

Controlling the Reader With KCjunior

Instructions for setting up and operating the ELx800 using KCjunior software.

Controlling the Reader With KC4

Instructions for setting up and operating the ELx800 using KC4 software.

Appendix B: Using 384-Well Geometry

KCjunior

Guidance on manipulating data from 384-well plates using KCjunior.

KC4

Guidance on manipulating data from 384-well plates using KC4.

Appendix C: Report Format

Samples with calls on Matrix Report

Shows a matrix report displaying sample results and calls.

Curve Fit Report

Illustrates the graphical representation of curve fitting results.

Samples with calls on Column Report

Displays sample results and calls in a columnar format.

Column Report without samples

Example of a column report showing only header and control data.

Panel Report

Shows a report detailing results for assays within a defined panel.

Assay Detail Report (Sheet 1 of 2)

Comprehensive report detailing assay parameters and settings.

Assay Detail Report (Sheet 2 of 2)

Continuation of the detailed assay parameters and settings report.

Appendix D: Comparison of the ELx800 and the EL800

Feature Comparison Table

A table comparing the features of the ELx800 and EL800 microplate readers.

Appendix E: Instructions for Programming a New Assay

Sample ANA Screen Enzyme Immunoassay Kit (Transformation and Cutoff)

Example of programming an assay with transformation and cutoff calculations.

Sample Human Anticardiolipin IgG Enzyme Immunoassay Kit (Standard Curve and Cutoff)

Example of programming an assay with standard curve and cutoff determination.

Bio-Tek ELx800 Specifications

General IconGeneral
BrandBio-Tek
ModelELx800
CategoryLaboratory Equipment
LanguageEnglish