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BMG Labtech FLUOstar OPTIMA User Manual

BMG Labtech FLUOstar OPTIMA
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Operating Manual
Revision I
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BMG Labtech FLUOstar OPTIMA Specifications

General IconGeneral
BrandBMG Labtech
ModelFLUOstar OPTIMA
CategoryCard Reader
LanguageEnglish

Summary

Technical Specifications

Measurement Principles

Lists measurement principles for FLUOstar, POLARstar, and LUMIstar OPTIMA.

Light Source

Details the high-energy xenon flash-lamp used in the OPTIMA instruments.

Detector

Specifies the side window, current type photomultiplier tube detector.

Filters

Describes filter wheels for FLUOstar/POLARstar and emission filter wheel for LUMIstar.

Gain Control

Explains software selectable and automatic gain adjustment.

Plate Carrier

Details the auto lock microplate carrier and supported formats up to 1536-well.

Reagent Injectors

Specifies up to two built-in injectors, volumes, and speed.

Shaking

Describes linear, orbital, and figure eight shaking options.

Incubation

Details incubation temperature range from ambient +8°C to 45°C, stability 0.2°C.

Shortest Reading Time

Lists shortest reading times for 96, 384, and 1536-wells.

Fluorescence Intensity

Details limit of detection and spectral range for fluorescence intensity.

Luminescence

Provides limit of detection, spectral range, and dynamic range for luminescence.

Time-Resolved Fluorescence

Specifies limit of detection, spectral range, and dynamic range for time-resolved fluorescence.

Fluorescence Polarization

Details limit of detection, spectral range, and dynamic range for fluorescence polarization.

Absorbance

Specifies spectral range, dynamic range, and reproducibility for absorbance measurements.

Computer Interface

Details the RS232 interface specifications, baud rate, and protocol.

Power Requirements

Outlines voltage, frequency, consumption, and fuse specifications.

Dimensions

Provides the height, width, and length of the instrument.

Weight

States the instrument's weight as 28 kg.

Ambient Conditions

Lists operating and storage temperature, and humidity limits.

Instrument Conformity

Specifies conformity standards for voltage category, contamination class, and protection class.

Robotic Capabilities

Mentions optional robotic capabilities like a stacker for 50 microplates.

Installation

Transport Pin

Explains how to unlock the transport pin after moving the instrument to its permanent location.

Power and Communication Connections

Details power connection, voltage check, and RS232 cable setup for PC communication.

Instrument Overview

Front View

Identifies key components like the reagent box, plate in/out button, and indicator lights.

Back View

Identifies rear panel components: power switch, connectors, and serial number.

Description of Components

Optics

Discusses UV/Vis optics for top reading and available optics for various applications.

Installation and Changing of Optics

Explains the quick-fix mounting system for easy optics exchange without tools.

Combination Optics (Fluorescence Intensity and Absorbance)

Details the combination optic with light guides for fluorescence and absorbance measurements.

Fluorescence Intensity Optics

Describes liquid-filled light guides for fluorescence and time-resolved fluorescence measurements.

Absorbance Optics

Mentions an optimized optic for absorbance mode and the use of the combination optic.

Luminescence Optics

Describes the luminescence optic, its silver light guide, and two dedicated versions.

Fluorescence Polarization Optics

Explains light guides for polarization and the required orientation of the measurement head.

Dual Emission Optics

Details dual emission optics for simultaneous measurement of two wavelengths, requiring two PMTs.

High Density Optics

Describes high-density optics for minimizing crosstalk in 384- and 1536-well plates.

Bottom Optics

Discusses bottom optics used for fluorescence, luminescence, and absorbance measurements.

384-Well Injection Head

Explains the special injection head for 384-well plates, requiring bottom reading.

Filters

Details factory-installed filters and the need to type them into the filter table.

Filter Change and Installation

Provides instructions on filter installation direction, referencing an arrow on the filter.

Fluorescence Filters

Discusses bandwidth variations for fluorescence filters based on fluorophore types.

Fluorescence Polarization Filters

Explains filter positioning (180° apart) for fluorescence polarization measurements.

Dual Emission Filters

Details filter positioning (180° apart) for dual emission measurements.

Absorbance Filters

Describes filter selection for absorbance, including 'empty' option for direct passage.

Luminescence Filters

Discusses bandwidth and optimization of luminescence filters for different assays.

Spacers

Explains the use and installation of 2mm height spacers for microplate height adjustment.

Reagent Injectors

Details the reagent injectors, their materials, and care instructions.

Use and Maintenance of the Reagent Injectors

Provides guidelines for using, cleaning, and maintaining reagent injectors.

Instrument Disinfection

Disinfection Steps

Lists step-by-step instructions for disinfecting the instrument using formaldehyde and alcohol solutions.