BMG Labtech FLUOstar OPTIMA - Technical Specifications; Measurement Principles; Light Source; Detector

BMG LABTECH FLUOstar / POLARstar / LUMIstar OPTIMA Operating Manual
2007-11-19 0413B0001I 5/23
1 Technical Specifications
Measurement principles: FLUOstar OPTIMA:
- Fluorescence intensity
- Time-resolved fluorescence
- Luminescence (opt.)
- Absorbance (opt.)
- Upgradeable to POLARstar OPTIMA
POLARstar OPTIMA:
- Fluorescence polarization
- Simultaneous dual emission fluorescence
- Fluorescence intensity
- Time-resolved fluorescence
- Luminescence (opt.)
- Simultaneous dual emission luminescence (opt.)
- Absorbance (opt.)
LUMIstar OPTIMA:
- Luminescence
- Simultaneous dual emission luminescence (opt.)
- Upgradeable to FLUOstar / POLAstar OPTIMA
Light source: FLUOstar OPTIMA & POLARstar OPTIMA: - High-energy xenon flash-lamp
Detector: Side window, current type photomultiplier tube
Filters: FLUOstar OPTIMA & POLARstar OPTIMA: - 2 filter wheels: with 8 excitation
and 8 emission filter positions
LUMIstar OPTIMA: - Emission filter wheel with 8 filter positions
Gain control: Software selectable gain
Automatic gain adjustment
Plate Carrier: Auto lock microplate carrier.
All microplate formats up to 1536-well in all detection modes.
Microplates should fulfil the SBS specification and non-SBS formats should fit:
(lxwxh) (mm) max: 128x86x20; min: length 124.
Reagent injectors: Up to two built-in reagent injectors
Individual injection volumes for each well
Injection volumes definable down to 3µl
Up to 4 independent injection actions per well
Variable injection speed (100 µl/s to 420 µl/s)
Shaking: Linear, orbital and figure eight shaking
Programmable shake time and diameter
Incubation: Incubation range from ambient +8°C to 45°C, in 0.1°C steps
Extended incubation up to 60°C (optional)
Temperature monitoring (without incubation)
Temperature stability 0.2°C
Shortest reading time: Fluorescence intensity: - 15 s for 96-wells
- 30 s for 384-wells
- 57 s for 1536-wells
Luminescence: - 20 s for 96-wells
- 55 s for 384-wells
Fluorescence intensity: Limit of detection 0.9 fmol / well fluorescein
Spectral range (ex. and em.): 240 ... 740 nm
Dynamic range 8 decades