Step # 10 “My Instrument” Software can now be installed from the Buck M500 CD like a normal
Windows program. Start, Run, then Browse to find the command line which is normally D:\
BuckM500\setup.exe. Do not change the “MY Instrument” default directory.
Step # 11 Before you enter GRAMS/AI please turn off your screen savers or increase the activation
time so that it will not activate during the scans. (It can look very confusing!) Next make sure the
M500 is plugged into COM 1 or 2 (3 & 4 are not recommended) and that it has had a suitable warm up
time.
To start GRAMS/AI double click the GRAMS/AI’s Icon. The first time you go into MyInstrument the
software will ask for your COM port. Pick the correct COM # (1, 2, 3 or 4) If you make a mistake the
“buck500.ini” can be changed with notepad.exe.
To store you first reference scan that will later be used to change your sample to double beam, choose
Collect, (MYBUCK if more than one instrument), Scan Sample. You will then watch a single beam
real time auto averaging of the scan on the screen. When it is done it will change from Page 2
MYBUCK to Page 1 GRAMS/AI. If the scan looks OK pick File, Save Trace As …, and choose a name
and save location for the file. Then choose File, Close Trace, Current Trace to close this saved file.
Now run your sample the same way and depending on the requirements save the file like the reference
scan or just convert to double beam. Change the scan to double beam by Application, Y-Axis
Conversion and then pick Transmission or Absorbance. You will have to pick the reference scan you
did before the sample and click Done. If the scan is good do the Save Trace As … like above. You will
now have an auto scaled double beam run. When scans are run they are just under the name
BUCKDEMO.SPC if you do not change the name the next scan that is run will erase it.
The print landscape mode gives a more normal looking hard copy. If you have problems please call
from in front of the working M500 and computer for the fastest support. The M500 data collection can
be checked with just running the MYBUCK software alone. The GRAMS/AI is needed for all data
manipulation. Do not multi-task programs while running the MYBUCK software.
If the “Scan Sample and Instrument Setup” doesn’t show up under “Collect” or there is no “Collect”
see trouble shooting Section O.
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