Operation of the BUCK M500 / GRAMS/AI Software System
Basic and Advanced Operation
1. Collection of Data & Generation of Spectrum:
Turn on M500 30 minutes before analysis.
Turn on computer and printer. Access Start, Programs, Galactic and double click GRAMS AI.
Click thought the boxes until to Page 1 of GRAMS/AI.
Click on "Collect", ("MYBUCK" if more than one Instrument), “Instrument Setup” and check
default: START: 4000 END: 600 WN: 4000 PEN: 0.1 ORD SCALE: %T
SCAN TYPE: Reference GAIN: 1 for Prism Cell or 0 for direct scan SCAN TIME: 3mins
Make any changes, as needed.
Make sure the clean Prism Cell or empty cell holder is in place, click on "Scan Now” (red box
in this window). System will show real-time display of Background Single-Beam spectrum.
When BKG Scan is completed, go to “FILE” and “SAVE TRACE AS…” and give this
Reference Spectrum a name.
Place Sample in holder, click on “Collect”, (“MYBUCK” if more than one Instrument) and
"Instrument Setup". Click on “Select” and load in this Reference Spectrum file, then click on
“Scan Now”. System will show real-time display of Sample Single-Beam (check for peaks in
H-C region to verify a good Sample thickness or ATR contact), then AUTOMATICALLY
generate the ratio’d %T Spectrum.
NOTE: Some computers may experience “data faults” if other applications are accessed
and multi-tasked during the Data COLLECTION cycle. Wait for the GRAMS
to return to the “Page-1” screen to access the toolbars or other programs.
At the end of the Sample scan use “FILE” and “SAVE TRACE AS…” to assign this Sample
scan a name and location of your choice.
Click on "File", " Save Trace As " and enter in file name for this sample. It will be written to
disk and saved for future reference.
2. Spectral Smoothing: (for ATR spectra, water vapor noise, KBr pellets)
With the Sample scan on screen, click on "Applications", "Smooting”, click on "Sav-Golay".
Highlight the "Points" number and enter in "11" (typical range is 5-49 points, ranging from
sharper peaks to smoother backgrounds), then go down to bottom of this screen and click on
"Apply" if it looks good click “OK” if not choose a higher or lower number.
System will then display a SMOOTHED scan and ask you for file status. Click on "ADD
NEW" and file will be temporarily saved as SMOOTH.
3. Removing Carbon Dioxide Bands from SCAN: (for COSMETIC use only!)
Click on "Applications", "Utilities" then “Zap”, the top shows result, right click on the bottom
screen. Drag the blue lines left and right until you like the result in the top box..
Right-click on the bottom scan to create a “Zap” box, and adjust the edges of box with the Left
mouse buttons. Right-clicking on another portion of the scan creates additional “Zap” boxes.
The Result Screen will show what the corrected spectrum looks like after the "Zap". If
acceptable, click "OK" and "ADD NEW" to save file temporarily as ZAP.
Click on the yellow, 4-point arrow icon to auto-scale the corrected spectrum.
4. Baseline Correction: (for bad KBr pellets, uneven ATR scans)
With the Zap scan on screen, click on "Applications"and choose "Baseline Correction" then
click on "Multi-Point" option.
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