10
8 TROUBLESHOOTING
If there is a leak or the pipette is not accurate,
check for the following:
• Theconnectingnutisloose.
Tighten the connecting nut.
• Thetip-holderiscrackedorscored.
Remove the tip ejector and inspect the tip-holder.
For F2 to F20 models, if the tip-holder is damaged,
the piston may also be damaged. Replace the dam-
aged parts (see Chapter 9). When re-assembling the
pipette, the connecting nut should be hand tightened.
• Chemicaldamagetothepistonandseals.
Replace the piston and seals (see Chapter 9). Wash
the inside of the tip-holder using distilled water.
• Improperreassembly.
Disassemble the pipette and reassemble the pipette
correctly.
If there is liquid in the tip-holder, clean the pipette
as follows:
• Removethetipejector.Unscrewtheconnecting
nut and rinse the tip-holder, piston, seal and O-ring
with distilled water. Dry these parts and reassemble
the pipette.
If necessary, the tip-holder, connecting nut and the
tip ejector can be autoclaved for 20 minutes at 121°C
and at 0.1 MPa pressure. The seal and the O-ring
should not be autoclaved.
If there is an air bubble when the sample is
aspirated:
• Dispensethesampleintoitsoriginalvessel.
• Ensurethatthetip isproperlyimmersedinthe
sample liquid.
• Aspiratethesamplemoreslowly.
If the bubble appears a second time, replace the tip.
The outside of the PIPETMAN Fixed can be cleaned
using a cloth dampened with isopropanol.