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Infors HT Minifors 2 User Manual

Infors HT Minifors 2
217 pages
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Operating Manual
2019-02-07
Minifors 2
Benchtop Bioreactor
Dok-ID: 75623 / V.03.00 - Original

Table of Contents

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Infors HT Minifors 2 Specifications

General IconGeneral
BrandInfors HT
ModelMinifors 2
CategoryLaboratory Equipment
LanguageEnglish

Summary

Minifors 2 Benchtop Bioreactor Operating Manual

General Information and Notices

About this Manual

Describes the manual's purpose, scope, and adherence to safety instructions.

Explanation of Special Notices

Explains signal words like DANGER, WARNING, CAUTION, and ATTENTION.

Equipment Identification Plate

Details the information found on the standard identification plate.

Declaration of Conformity and Customer Service

Lists EC directives compliance and provides customer service contact details.

Safety and Responsibility Guidelines

Intended Use, Incorrect Use and Misuse

Defines proper usage and prohibits misuse or applications outside intended scope.

Qualified Personnel Requirements

Specifies qualifications for providers, users, and operators.

General and Particular Hazards

Covers electrical, chemical, biological, and physical hazards.

Warning Symbols on Equipment

Lists warning symbols attached to the equipment for hazard identification.

Declaration of Decontamination

Outlines decontamination requirements for returning equipment.

Setup and Function of the Minifors 2 Bioreactor

Basic Unit Components Overview

Identifies key parts of the bioreactor's basic unit.

Main Switch, LED Strip, and Pumps

Details the main switch, status LED strip, and peristaltic pumps.

Connection Ports and Interfaces

Describes power, water, gas, signal, and motor cable connections.

Culture Vessel and Top Plate Configuration

Explains the culture vessel, its holder, and top plate with port details.

Temperature Control System

Details the thermal block and adapter system for temperature regulation.

Stirrer and Gassing System

Describes the stirrer mechanism and the gas supply system.

pH, pO2, and Antifoam Control Systems

Explains the measurement and control systems for pH, pO2, and foam.

Optional Equipment and Accessories

Turbidity Measurement System

Describes the optional Optek ASD12-N turbidity sensor and its calibration.

Exit Gas Analysis System

Details the optional system for analyzing exit gases using CO2 and O2 sensors.

Key Accessories Overview

Lists and describes essential accessories like plugs, adapters, sensors, and filters.

Sampling System - Super Safe Sampler

Explains the operation and aseptic sampling procedure.

Transport, Storage, and Installation

Transport Safety Instructions

Provides guidelines for safe internal and external transport of the equipment.

Storage Conditions

Specifies requirements for storing the equipment and its components.

Installation and Initial Operation Steps

Covers location requirements, distances, and connecting supply lines.

Test Run Procedures

Guides through performing initial tests for cooling, stirring, heating, and gassing.

Before Cultivation Procedures

Vessel Preparation and Autoclaving

Details checking gaskets, mounting components, filling, and autoclaving the vessel.

Connecting Culture Vessel and Preparing for Cultivation

Covers hanging the vessel, fitting pump heads, filling hoses, and connecting systems.

Cultivation Process Steps

Preparing the Medium

Explains warming the medium and setting parameters before inoculation.

Sampling Procedures

Describes how to take samples from the culture vessel using the Super Safe Sampler.

Inoculation Methods

Details inoculation via syringe or dip tube/addition port adapter.

Harvesting and Vessel Emptying

Covers harvesting the culture and emptying the vessel and hoses.

Post-Cultivation Autoclaving

Outlines the procedure for autoclaving the vessel after cultivation.

Operation and Control Interface

Screen Areas, Menu Navigation

Overview of the main screen layout, menus, and control elements.

System Settings Configuration

Guides through VESSEL TYPE, APPEARANCE, NETWORK SETTINGS, and USB menus.

Parameter Groups and Functions

Explains parameter groups (MAIN, EXTENDED, EXIT GAS, PUMPS) and functions.

Setting Setpoints and Parameter Alarms

Details how to adjust parameter setpoints and configure alarms.

Cascade Control Configuration

Explains how to link parameters for automated control like pO2.

Pump Configuration and Operation

Details configuration for Pumps 1-4 and AUTO FILL/EMPTY functions.

Sensor Calibration Procedures

Provides step-by-step guides for calibrating pH, pO2, and Turbidity sensors.

PID Controller Principles and Settings

Explains PID control basics, setting values, and adjustment tips.

Alarm Management

Describes how parameter alarms and equipment errors are displayed and managed.

Cleaning and Maintenance Procedures

Cleaning Agents and Routine Cleaning

Lists cleaning agents and describes routine cleaning of the culture vessel.

Disassembly for Cleaning

Details the removal of components like top plate, sensors, hoses, and filters.

Cleaning Specific Components

Covers cleaning sensors, hoses, pump heads, sampling systems, and exit gas coolers.

Basic Unit, Operating Panel, and Maintenance Plan

Describes cleaning the main unit and outlines the maintenance schedule.

Decalcifying the Equipment

Explains the process for removing calcium deposits from the temperature control system.

Troubleshooting Interferences

Basic Unit and Operating Panel Issues

Lists common problems with the main unit and display, and their solutions.

Drive System and Motor Interferences

Addresses issues related to the stirrer motor not starting or operating erratically.

Temperature, Gassing, and pH System Interferences

Covers problems related to temperature control, gassing, and pH.

pO2 and Antifoam System Interferences

Lists common issues with pO2 control and antifoam systems.

Feed Solution and Repair Return

Addresses feed pump issues and procedures for returning equipment for repair.

Disassembly, Disposal, and Technical Data

Equipment Disassembly and Disposal

Provides guidelines for environmentally friendly disassembly and disposal.

Equipment Dimensions and Vessel Sizes

Presents physical dimensions of the bioreactor and culture vessels.

Connection Requirements and Specifications

Details electrical, water, gas connections, and component specifications.

Stirrer, Temperature, Gassing, and pH Specifications

Lists technical specifications for stirrer, temperature, gassing, and pH systems.

pO2, Antifoam, Pumps, and Operating Conditions

Provides specifications for pO2, antifoam, pumps, and operating environments.

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