Integra MEDIACLAVE 10 - Initial Setup and Configuration; Language Selection; Media Preparation Programs; Preparing the Cuvette and Program

2/6 136968_V01_QSG_MEDIACLAVE_10_30_EN.docx
Language selection
Navigate to SYSTEM PARAMETER and LANGUAGE SELECTION. Select a language and press
SAVE. Set other system parameters to your requirements.
Preparing a media preparation program
• Check, if the white pivot pin disk inside the cuvette is pushed fully downwards and place the
magnetic stirrer bar on the pivot pin inside at the cuvette bottom.
• Place the cuvette in the instrument vessel.
• Using both grips, turn the cuvette approx. 2 cm
clockwise until the bolt locks into position as shown
beside.
• To remove the cuvette, turn it counter clockwise and lift it up.
Fill coupling water
• Prepare softened coupling water: Add a cup of tap water or a pinch
of salt to distilled water to make it conductive, which is required for
the level sensors.
• Navigate to MAINTENANCE, select DRAINING/FILLING and press
FILL COUP. WATER.
•
Fill the vessel with coupling water until the upper level sensor is
covered (approx. 2.7 l for MC10, 8.5 l for MC30).
Select a program
• Navigate to SELECT PROGRAM. The first programs are already pre-defined with default values:
Operation mode
Description
STANDARD
For preparation and sterilization of media
CHOCOLATE AGAR
Two step program for preparation of complex media.
AUTOCLAVE
For sterilization of media in glassware (MC10 only)
WATER BATH
For pre-swelling, warming up and thermostatting media
• Select a program to be defined using the arrow keys and press SELECT PROGRAM and
PROGRAM SETTINGS in order to adjust the program.
• Using the arrow keys, select a parameter you wish to change. Press CHANGE and follow the
information on the screen.
Run a STANDARD or CHOCOLATE AGAR program
The nominal volume of the cuvette (10/30L) must not be exceeded. Consider vortex, foam
or bubble formation and swelling.
• Navigate to SELECT PROGRAM. Select the previously defined STANDARD or CHOCOLATE
AGAR program. Add water and ingredients according to your recipe into the cuvette. Press
START and follow the instructions on the screen.
The semi-rigid temperature probe (MC10) must be handled with great care and must not
be deflected more than 30 degrees from the vertical! Do not clamp the cable of the flexible
temperature probe when closing the vessel lid.