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Leica BIOSYSTEMS HistoCore SPECTRA CV - User Manual

Leica BIOSYSTEMS HistoCore SPECTRA CV
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HistoCore SPECTRA CV
Coverslipper
Instructions for Use
English
Order No.: 14 0514 80101 - Revision L
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 2.2, Revision L - 02.2022

Table of Contents

Questions and Answers

Summary

Important Notes

Symbols and Their Meanings

Explains symbols used in the manual and their meanings, including warnings and notes.

Regulatory and Identification Symbols

Details various symbols like CE, UKCA, CSA, RoHS, WEEE, and article numbers.

Instrument Identification and Safety Symbols

Explains symbols for serial number, instructions, UDI, power status, electric shock, crushing, flammability, and laser.

Transport and Handling Symbols

Covers symbols related to transport conditions like IPPC, country of origin, fragility, storage, stacking, and temperature.

Instrument Type and User Group

Specifies applicable instrument type and defines the authorized user group and their requirements.

Intended Purpose and Copyright

Describes the instrument's intended use and outlines copyright information for the software.

Safety

Safety Notes and Warnings

Details critical safety precautions, protective devices, residual risks, and general warnings.

Hazard Warnings

Explains hazard warnings, including malware, laser radiation, and general markings on the instrument surface.

Transport, Installation, and Reagent Handling Safety

Covers safety aspects for transport, installation, and handling of reagents and solvents.

Operating the Instrument Safely

Provides safety instructions for operating the instrument, including PPE and environmental controls.

Cleaning and Maintenance Safety

Details safety precautions to be observed during cleaning and maintenance procedures.

Instrument Safety Features

Explains safety features like hood interlocks and software restart requirements.

Instrument Components and Specifications

Standard Delivery and Packing List

Details the items included in the standard delivery package with their order numbers.

Technical Data

Provides detailed technical specifications including electrical, environmental, and connectivity data.

Physical Dimensions and Weights

Specifies the physical dimensions and weight of the instrument and workstation.

Performance Characteristics

Details performance aspects like usable slides, coverglass capacity, and mounting medium application.

Factory Settings

Lists default instrument settings for parameters like oven temperature, date, time, and language.

General Overview - Front View

Illustrates and labels the external components visible from the front of the instrument.

General Overview - Rear View

Illustrates and labels the rear view of the instrument, including connections.

General Overview - Inside View

Illustrates and labels the internal components and modules of the instrument.

Installation and Instrument Setup

Installation Site Requirements

Specifies the necessary environmental, electrical, and space requirements for instrument installation.

Electrical Connection

Provides step-by-step instructions for connecting the instrument to the power supply.

Internal Battery and UPS Usage

Explains the internal battery function and guidelines for using an external UPS.

Exhaust Air Connection

Guides on properly connecting the instrument to an external exhaust air extraction system.

Installing Accessories

Covers the installation of accessories like drawer inserts and the waste tray.

Filling and Inserting Needle Cleaning Container

Detailed steps for filling and correctly inserting the needle cleaning container.

Switching On and Shutting Down the Instrument

Explains the procedure for powering the instrument on, initialization, and shutting it down.

Refilling Consumables

Provides guidance on refilling essential consumables like mounting medium and coverglass.

Inserting Coverglass Cartridge

Detailed instructions for inserting a new coverglass cartridge into the instrument.

Inserting Mounting Medium and Prime Bottles

Instructions for installing the mounting medium bottles and the prime bottle.

Preparing and Inserting Reagent Vessels

Steps for preparing, filling, and correctly inserting reagent vessels into the load drawer.

Operation

User Interface Overview

Explains the main window, touchscreen interface, and key display elements.

Grayed Out Function Keys

Explains why function keys may be inactive and lists always possible operations.

Elements of the Status Display

Describes the meaning of various symbols on the status bar (date, time, battery, warnings, modes).

Process Status Display

Explains how to view the status of ongoing coverslipping processes and racks.

Consumables Management System (CMS)

Details the CMS for monitoring mounting medium, coverglass, and slide levels.

Displaying the Drawers

Shows how the status of load and unload drawers, including rack positions, is displayed.

Main Menu Overview

Provides an overview of the main menu options: Process Status, Module Status, Parameter Sets, Settings, User Settings.

Entry Keyboard

Explains the use of the on-screen keyboard for entering passwords and data.

User Settings and Access Levels

Allows configuration of user access levels (Standard, Supervisor, Service technician) and password management.

Basic Settings Configuration

Covers fundamental instrument and software settings like language and regional formats.

Language Settings

Allows selection and configuration of the instrument's display language.

Regional Settings (Date and Time)

Configures date and time formats, including 12/24 hour time display.

Date and Time Configuration

Details how to set the current date and local time using rollers.

Alarm Sounds Menu

Allows configuration of audible alarms and signal sounds for errors and notifications.

Oven Settings

Explains how to configure and manage the instrument's oven settings for slide drying.

Volume Calibration

Allows adjustment of mounting medium application volume by supervisors for optimal results.

Data Management

Covers exporting, importing, and updating instrument data, logs, and software.

Event View

Allows viewing of instrument log files, event history, and error messages.

Parameter Settings

Enables creation, modification, and assignment of parameter sets and rack handle colors.

Creating New Parameter Sets

Step-by-step guide to create new parameter sets for coverslipping operations.

Assigning Parameter Sets to Rack Handle Colors

Assigns colors to rack handles for parameter set identification and workflow management.

Properties of Mounting Medium

Information about Leica's validated mounting media and their advantages.

Properties of Coverglass

Details on Leica's validated coverglass and its automatic detection by CMS.

Adjustment of Application Volume

Allows adjustment of mounting medium application volume based on lab conditions.

Reagent Vessels in the Load Drawer

Notes on reagent vessel compatibility, fill levels, and proper storage to prevent evaporation.

Module Status

Displays the status of consumables and provides access to cleaning and priming options.

Daily Instrument Setup

Station Overview

Provides a visual overview of the instrument's main stations and components.

Switching On and Shutting Down the Instrument

Step-by-step guide to power the instrument on, including initialization, and shut it down.

Checking and Refilling Consumables

Details how the instrument checks consumables and provides instructions for refilling them.

Changing the Mounting Medium Bottle

Instructions for safely replacing the mounting medium bottle, including expiration date checks.

Monitoring and Refilling Needle Cleaning Container

How to check and refill the needle cleaning container with xylene.

Checking and Replacing Coverglass Cartridge

Guides on checking and replacing the coverglass cartridge, including CMS data import.

Emptying the Waste Tray

Procedure for checking and emptying the waste tray, including safety precautions.

Inspect Pick&Place Module

Instructions for inspecting and cleaning the Pick&Place module components.

Load Drawer Operations

Focuses on checking reagent vessel fill levels and handling the load drawer.

Unload Drawer Operations

Instructions for inspecting and cleaning the unload drawer.

Preparing the Rack

Instructions for preparing racks, including attaching colored handles correctly.

Brief Inspection Before Coverslipping

Pre-operation checks to ensure readiness for coverslipping, including levels and component status.

Starting the Coverslipping Operation

Guides on how to initiate the coverslipping process by inserting a prepared rack.

Monitoring the Coverslipping Operation

How to retrieve and monitor details of ongoing coverslipping operations.

Coverslipping Operation Finished

Describes what happens after the coverslipping operation is completed, including rack transport.

Removing Racks from the Unload Drawer

Instructions for safely removing completed racks from the unload drawer.

Pausing or Canceling Coverslipping Operation

How to pause an operation by opening the hood or cancel an active coverslipping process.

Workstation Operation Notes

Key considerations and warnings for operating the instrument in workstation mode with SPECTRA ST.

Starting Coverslipping in Workstation Mode

How to initiate the coverslipping process when operating in workstation mode.

Cleaning and Maintenance

Important Notes on Cleaning

Crucial safety and general guidelines before starting any cleaning or maintenance tasks.

Cleaning TFT Touchscreen

How to clean the instrument's touchscreen display using a lint-free cloth.

Cleaning Load and Unload Drawers

Procedures for cleaning the load and unload drawers, including reagent vessel removal.

Interior Cleaning Procedures

Detailed steps for cleaning the internal parts of the instrument, including drawers and rear areas.

Cleaning the Prime Bottle

How to clean the prime bottle if it contains dried residual mountant.

Cleaning Cannulas for Mounting Medium Bottle

Cleaning procedures for the cannulas attached to the mounting medium bottles.

Cleaning the Needle

Step-by-step guide for cleaning the instrument's needle, including soaking in xylene.

Filling and Changing Needle Cleaning Container

Procedures for filling and changing the needle cleaning container with xylene.

Removing Needle Cleaning Container Unit

How to remove the entire needle cleaning container unit if it is stuck due to mounting medium.

Cleaning the Pick&Place Module

How to clean the suction cups, skids, and sensor pin of the Pick&Place module.

Exchanging Suction Cups

Instructions for replacing deformed or dirty suction cups on the Pick&Place module.

Cleaning the Waste Tray

Procedure for cleaning the waste tray, including safety precautions for handling.

Cleaning Reagent Vessels

How to clean reagent vessels, including dishwasher compatibility and pre-cleaning steps.

Cleaning Racks and Handles

Cleaning instructions for racks and their handles, including xylene bath use.

Changing the Active Carbon Filter

How to replace the active carbon filter to reduce reagent vapors in the exhaust air.

Preparing Hose System for Priming and Cleaning

Steps to prepare the hose system for priming and cleaning operations.

Quick Prime Procedure

Details the Quick Prime rinsing program for ensuring proper mounting medium flow.

Extended Prime Procedure

Explains the Extended Prime program for ensuring no air in the hose system after bottle replacement.

Cleaning the Hose System

Procedure for cleaning the entire hose system using a cleaning solution (xylene).

Recommissioning After Transport or Storage

Steps to recommission the instrument after transport or long-term storage.

Recommended Cleaning and Maintenance Intervals

Outlines recommended schedules for daily, weekly, quarterly, and as-needed cleaning tasks.

Daily Cleaning and Maintenance Checklist

A comprehensive checklist of daily cleaning and maintenance tasks for various components.

Weekly Cleaning and Maintenance Checklist

A checklist of weekly cleaning and maintenance tasks for components like the needle and touchscreen.

Quarterly Cleaning and Maintenance

Tasks for quarterly cleaning and maintenance, including active carbon filter replacement.

Cleaning and Maintenance as Necessary

Situational cleaning and maintenance tasks, such as for broken glass or spilled media.

Malfunctions and Troubleshooting

Troubleshooting Common Issues

Lists common problems like black screen, needle container issues, and drawer sticking with causes and corrections.

Coverglass and Air Bubble Issues

Addresses problems like intact coverglass in waste tray and air bubbles between specimen and coverglass.

Mounting Medium Application Problems

Troubleshooting for insufficient or excessive mounting medium application and damaged specimens.

Pick&Place Module and Coverglass Fall Issues

Addresses issues with the Pick&Place module, suction cups, and moist coverglass magazines.

Needle and Mounting Medium Errors

Troubleshooting for needle issues, 'mounting medium not detected' errors, and oven temperature problems.

Power Failure Scenario and Instrument Failure

Describes actions to take in case of power failures, software crashes, or instrument malfunctions.

Manual Rack Removal Procedures

Step-by-step instructions for manually removing a rack during instrument malfunctions.

Malfunction at Coverglass Receptacle

Addresses issues with the Pick&Place module losing or failing to pick up coverglass.

Removing Rack from Elevator

Procedures for removing a rack from the elevator of the coverslip line during malfunctions.

Removing Rack from Oven or Behind Oven

Steps to safely remove racks from the instrument's oven area.

Removing Rack from Rotator

Instructions for removing a rack from the instrument's rotator.

Removing Rack from Transport Arm Gripper

Steps to remove a rack from the transport arm gripper.

Rack Removal from SPECTRA ST Transfer Station

How to handle racks during workstation transfer malfunctions between ST and CV.

Replacing Main Fuses

Safety precautions and steps for replacing the main fuses in the instrument.

Optional Accessories and Consumables

List of Optional Accessories

Lists various optional accessories like racks, handles, bottles, filters, and tools with order numbers.

Exhaust Hose and Active Carbon Filter

Details on exhaust hose sets and active carbon filters, including order numbers.

Waste Tray and Needle Cleaning Container

Information on waste trays and needle cleaning containers with their order numbers.

Racks and Handle Colors

Details on racks for 30 slides and available colored handles for parameter set assignment.

Prime Bottle Assembly

Information on the prime bottle assembly, consisting of bottle, cap, insert, and O-ring.

Cleaning Bottle and Suction Cups

Details on cleaning bottles and individual suction cups with their order numbers.

Storage Rails and Tool Kit

Information on storage rails for the unload drawer and the tool kit contents.

Cut-Resistant Gloves and Rack Adapter

Details on cut-resistant gloves and the rack adapter for Stainers ST5010/ST5020.

Consumables Information

Lists mounting medium (Spectra X1) and coverglasses (Spectra CV premium) with order numbers.

Warranty and Service

Warranty Terms and Preventive Maintenance

Guarantees product quality and recommends yearly preventive maintenance by a qualified technician.

Service Information and Contact

Provides information needed for technical support calls, including model, serial number, and contact person.

Decommissioning and Disposal

Disposal Regulations and Disinfection

Guidelines for disposing of the instrument and contaminated objects according to local regulations.

Reducing Contamination and Contacting Representative

Requirements for thorough disinfecting before decommissioning or disposal and contacting Leica.

Decontamination Certificate

Certificate Requirements for Returns

Information about the required decontamination certificate for product returns and servicing.

User Responsibility for Returns

User responsibility for products returned without a certificate or as potential hazard sources.

Leica BIOSYSTEMS HistoCore SPECTRA CV Specifications

General IconGeneral
BrandLeica BIOSYSTEMS
ModelHistoCore SPECTRA CV
CategoryLaboratory Equipment
LanguageEnglish

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