2
3.2 Eyepiece
The eyepiece is secured to the eyepiece tube. Binocular viewing
bodies do not utilize set screws to secure the eyepieces.
3.3 Substage Condenser
The Leica BM E Compound Microscope is equipped with one of
two available substage condensers:
• 0.65 N.A. condenser, a five-position aperture disc diaphragm
which is color coded to match the objective markings.
• 1.25 N.A. (fixed) Abbe Condenser on a spiral mount
4.0 Operation
CAUTION: The top collector lens of the illumination system
in the base of the microscope may become warm or hot.
Avoid touching the lens directly.
The Leica BM E mroscope should always be used on a hard, stable
and level surface such as a laboratory table or work bench.
4.1 Illumination
4.1.1 Built-in Illumination System
The Leica BM E Compound Microscope should only be plugged
into a suitably grounded electrical outlet.
To turn on the electrical power, flip the switch located on the back
left of the base of the BM E. If you have the Tungsten-Halogen
model, the intensity control which is located on the left side of the
base should be turned all the way down before turning the
instrument off.
4.1.2 External Illuminator and Mirror
Position the external illuminator approximately 5cm (2”) in front of
the mirror.
NOTE: Not all Leica BM E Compound Microscopes are equipped
with a mirror. While looking through the eyepiece adjust the mirror
to provide the brightest and most evenly illuminated field-of-view.
4.2 Stage Preparation
Lower power objectives have a greater depth of field and are
generally used for initial focusing and viewing. The lower the
objective power, the greater area of specimen surface will be seen
in the field of view.
Before placing a specimen on the stage, rotate the nosepiece until
the lowest power objective is in the viewing position.
Be certain that the stage surface is free of dust, grit, or any other
material. These will scratch the slide or stage or may interfere with
the movement of a slide across the surface of the stage.
Place the slide under the stage clips with the cover glass facing
upward. If using a graduated mechanical stage, place the slide
between the stage fingers.
Position the specimen area of the slide over the center of the stage
aperture. When using a graduated mechanical stage, use the stage
control knobs to move the specimen slide.
Raise the stage by turning the coarse focus knob until the stage
reaches the upward stop. Do not view the slide through the eyepiece
at this point.
The Leica BM E Compound Microscope is ready to be focused.
4.3 Focusing
Using the 4x or 10x objective, focus the Leica BM E Compound
Microscope by looking through the eyepiece and turning the fine
focus knob. Continue to view the specimen through the eyepiece
until a crisp, clear image appears.
Adjust the aperture disc diaphragm condenser to match the
objective being used to obtain the clearest possible image. Adjust
the fine focus knob to sharpen the image to the center of the field
of view.
If using the 1.25 N.A. Abbe condenser, focus by moving it up and
down in its spiral sleeve to the point at which the field is most evenly
illuminated. Adjust the iris diaphragm by sliding the control lever to
obtain the clearest image.
The position of the aperture must be reset when changing to higher
power objectives. As magnification increases, the aperture
diaphragm must be opened.
4.4 Examining Specimen at High Power
When examining a specimen at high magnification, position the
slide so the feature is centered in the field of view. If the feature
you want to observe appears to the left of the field of view, move
the slide left to center it. If the feature you want to observe appears
to the right of the field of view, move the slide to the right.
By rotating the nosepiece, bring the next higher power objective
into viewing position. The objective is properly aligned when the
nosepiece clicks into place.
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