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4.5 Oil Immersion Technique
To utilize the full numerical aperture of an immersion objective (with
inscription “oil”), oil is added to both the objective and specimen
using the following procedure:
1. Focus on the specimen with a lower power objective (40x).
2. Put a drop of immersion oil on the specimen slide.
3. Turn the nosepiece to bring the immersion objective into
the light path, and focus with the fine adjustment knobs.
• Care should be taken to prevent bubbles from forming in
the oil, since they will deteriorate the lens performance.
Simply reapply the immersion oil if bubbles form.
IMPORTANT: After each use of the 100x objective, use a lens tissue
or soft cloth to wipe off all traces of oil from the objective and the
slide.
5.0 Care of the
Microscope
5.1 General
It is important to keep all optical components clean to obtain the
best optical performance. Keep a dust cover on the microscope
when it is not in use.
The objectives are easily subjected to dirt, dust and oil. Whenever
lack of contrast, cloudiness or poor definition is encountered,
carefully check the condition of the front objective lens with a
magnifier. To assure all objective lenses are clean, periodically
examine them with a magnifying glass.
If an optical surface becomes badly coated with dust or dirt, use
an air-filled syringe or soft brush to remove it before wiping the
surface clean.
IMPORTANT: Clean optical surfaces with a lint-free cloth, lens tissue
or a cotton swab moistened with methanol or commercially
available glass cleaner. Avoid excessive use of solvents. Interior
surfaces can be loosened or badly damaged if solvents seep in
around the lenses.
The higher magnification objectives use a small front lens. The
surface of this front lens can be cleaned with a small cotton swab.
Dampen the cotton tip with methanol and squeeze it dry. Wipe the
front lens lightly. Make sure that the cotton tip contacts the lens
surface. Check the objective with a magnifier after the cleaning.
IMPORTANT: If it is necessary to remove the viewing body, DO
NOT TOUCH the outer surface on the underside of the viewing body.
Finger prints on this surface will reduce the image clarity. This lens
can be cleaned in the same manner as objectives and eyepieces.
5.2 Mechanical Maintenance
All non-optical components of the Leica BM E Compound
Microscope can be cleaned with soap and water. Avoid using all
other harsh solvents or acetone-based products when cleaning.
The Leica BM E Compound Microscope requires only routine
maintenance. Neither the focusing mechanism nor the mechanical
stage require lubrication. Contact your Leica customer service
representative for more information.
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