Reagents
Sterilizer, Cleanser, and Debubbler
These recommended reagents have been formulated to optimize the performance of the FlowSight
®
System seals,
valves, syringes, and lines. The use of the recommended reagents is required for proper operation of the instrument.
The Sterilizer, Cleanser, and Debubbler reagents are used in the Sterilize and Debubble scripts.
Reagent Name Source Catalog#
Sterilizer 0.4-0.7% Hypochlorite VWR JT9416-1
Cleanser
Coulter Clenz
®
Beckman Coulter 8546929
Debubbler 70% Isopropanol Fisher Scientific A459
Sheath
D-PBS Ca
++
Mg
++
free
GE Healthcare Life
Sciences
SH3002803
6506-1L
(10X)
Rinse
Milli-Q Deionized water, at least 0.22 µm filtered (use mµ for
µm)
Varies
Calibration
Beads
FlowSight Calibration Beads Luminex 400300
Waste Fluid
The two liter waste bottle holds all of the fluids that have been run through the FlowSight
®
System. Add 200 mL of bleach
to the empty waste tank. It is recommended that the waste bottle contain 10% bleach when full.
Sheath Fluid
Two bottles are provided: one labeled Sheath to be filled with phosphate buffered saline (PBS with no surfactants) for
running samples and one labeled Rinse to be filled with de-ionized (DI) water for rinsing the instrument during shut-
down. Fluid is drawn from these bottles into the sheath and flush syringe pumps. The sheath pump helps to control the
speed of the core stream and the size of the core stream diameter. The flush pump is used to clean and flush the sys-
tem.
Fluid Level Warnings
Sensors behind the bottles trigger a pop-up window to alert the user that the volumes are low or the waste volume is
high. The triggers are set such that any script currently running can finish. Do not remove a bottle during the script. Once
the script has finished, remove the bottle and fill the appropriate solution. Waste can be emptied at any time. Dispose of
the waste in accordance with local regulations.
For Research Use Only. Not for use in diagnostic procedures.
15
Amnis
®
FlowSight
®
Imaging Flow Cytometer User Manual
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