Chapter 4: Troubleshooting
This section is designed to help you troubleshoot the operation of the FlowSight
®
System. If additional assistance is
required, contact the Luminex Technical Support.
System
l see Unstable fluidics (Air or clog in system)
l see Fluidics respond sluggishly
l see Event rate slows over time
l see Troubleshooting
l see Cross-contamination from previous samples
l see Erroneous fluid level indicator
l see Instrument will not pass ASSIST
l see Compensation fails to compute values and save data
Software
l see INSPIRE™ appears to freeze
l see INSPIRE™ fails to launch
l see Plots fail to update, or update slowly
l see Troubleshooting
Image
l see No Images
l see Imaging and acquisition rate is erratic, or appears frozen
l see Objects appear streaked
l see Objects are not centered in the channel
l see Objects are rotating in the core stream
l see Objects are out of focus
l see Objects are cropped
l see The two brightfield images are not of the same cell
l see Images appear pixelated or larger than normal
l see Not all 12 channels are being displayed
l see Troubleshooting
For Research Use Only. Not for use in diagnostic procedures.
42
Amnis
®
FlowSight
®
Imaging Flow Cytometer User Manual
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