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Molecular Devices QPix 420 User Manual

Molecular Devices QPix 420
225 pages
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5031496 G
August 2022
QPix 420
Colony Picking System
User Guide

Table of Contents

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Molecular Devices QPix 420 Specifications

General IconGeneral
Picking SpeedUp to 3000 colonies/hour
Power Supply100-240 VAC, 50/60 Hz
SoftwareQPix Software
Plate Types96-well, 384-well, 1536-well
Operating SystemWindows
TypeAutomated Microbial Colony Picker
LightingLED-based lighting system

Summary

Safety Information

Warnings, Cautions, Notes, and Tips

Explains user-attention statements and their meaning (Warning, Caution, Note, Tip).

Symbols on the Instrument

Lists and explains various safety symbols used on the instrument.

Before Operating the Instrument

Lists prerequisites for operating the instrument, including training and SDS review.

Electrical Safety

Details precautions to prevent electrical injuries and property damage.

Chapter 1: Introduction

Functionality of the QPix 420 System

Describes the system's features for colony picking, imaging, and sample handling.

Picking Microbial Colonies

Explains the process of automatically picking microbial colonies using the system.

Filters

Lists the filter options supported by the QPix 420 system for imaging.

Chapter 2: QPix 420 System Instrument Overview

Front Panel Controls and Display

Identifies and describes the controls and indicators on the instrument's front panel.

Instrument Connections

Details the power, data, and compressed air connection ports on the instrument.

Compressed Air Supply

Explains the requirement and connection of compressed air for the instrument.

Using Barcodes

Specifies requirements for using barcodes for plate identification and tracking.

Chapter 3: QPix 420 Software Overview

New Process Options

Lists various process types available, such as Gridding, Picking, and Replication.

Menu Options

Details the options available in the File, View, and Tools menus of the software.

QPix Utility Processes

Describes utility processes like Manage Sanitise Profiles and Camera Alignment.

Chapter 4: Starting and Setting Up the Instrument

Start-Up and Shutdown Procedures

Provides step-by-step instructions for powering on and shutting down the instrument.

Preparing to Run Processes

Outlines essential cleaning and setup procedures before running any process.

Changing the Head

Provides instructions for safely exchanging the picking head using the software.

Chapter 5: Sanitizing the Instrument Interior

Running Sanitise Processes

Explains how to run the Sanitise process to clean picking pins.

Running UV Sanitise Processes

Details the procedure for using UV light to sanitize the instrument interior.

Creating and Editing Sanitise Profiles

Guides users on creating and modifying Sanitise profiles for application needs.

Chapter 6: Picking Processes

Creating and Editing Picking Processes

Outlines the general procedures for creating and editing picking processes.

Opening the Picking Window

Instructions to launch the picking process interface in the software.

Selecting Picking Routines

Guides users on selecting or creating picking routines for execution.

Chapter 7: Blue-White Picking Processes

Creating and Editing Blue-White Picking Processes

Outlines procedures for creating and editing Blue-White picking routines.

Selecting Barcode Options

Details barcode options for Blue-White picking routines.

Setting Destination Plate Options

Configures destination plates for Blue-White picking.

Chapter 8: Zone of Inhibition Detection Picking Processes

Creating and Editing Zone of Inhibition Detection Picking Processes

Outlines procedures for creating and editing Zone of Inhibition picking routines.

Selecting Barcode Options

Details barcode options for Zone of Inhibition detection.

Setting Destination Plate Options

Configures destination plates for Zone of Inhibition detection.

Chapter 9: Regional Picking Processes

Creating and Editing Regional Picking Processes

Outlines procedures for creating and editing regional picking routines.

Selecting Barcode Options and Filter Pairs for Fluorescent Imaging

Details barcode and filter settings for regional picking.

Setting Destination Plate Options

Configures destination plates for regional picking.

Chapter 10: Blue-White Regional Picking Processes

Creating and Editing Blue-White Regional Picking Processes

Outlines procedures for creating and editing Blue-White regional picking routines.

Selecting Barcode Options

Details barcode options for Blue-White regional picking.

Setting Destination Plate Options

Configures destination plates for Blue-White regional picking.

Chapter 11: Control Plate Creation Processes

Creating and Editing Control Plate Creation Processes

Outlines procedures for creating and editing control plate creation routines.

Selecting Barcode Options

Details barcode options for control plate creation.

Setting Destination Plate Options

Configures destination plates for control plate creation.

Chapter 12: Manage Regional Tray Processes

Create New Regional Trays

Guides users in defining new custom plate receptacles for regional picking.

Modifying Regional Trays

Explains how to modify, copy, or delete existing regional tray definitions.

Chapter 13: Replication Processes

Opening the Replication Window

Instructions to launch the replication process interface.

Selecting Replication Routines

Guides users on selecting or creating replication routines.

Selecting Barcode Options

Details barcode options for replication routines.

Chapter 14: Rearraying Processes

Creating and Editing Rearraying Processes

Outlines procedures for creating and editing rearraying routines.

Selecting Rearraying Routines

Guides users on selecting or creating rearraying routines.

Selecting Barcode Options

Details barcode options for rearraying routines.

Chapter 15: Gridding Processes

Creating and Editing Gridding Processes

Outlines procedures for creating and editing gridding routines.

Selecting Gridding Routines

Guides users on selecting or creating gridding routines.

Selecting Barcode Options

Details barcode options for gridding routines.

Chapter 16: Data Viewer Processes

Finding Data in the Database

Guides users on searching and retrieving data from the database.

Displaying the Settings for Routines

Explains how to view the detailed settings of a specific routine.

Working With Tags

Describes how to create, add, and remove user-defined tags for data organization.

Chapter 17: Maintenance

Doing Preventive Maintenance

Outlines daily, weekly, and semi-annual maintenance tasks.

Cleaning the Instrument

Provides instructions for cleaning the instrument interior and components.

Sonicating the Pins and Springs

Details the process of sonicating pins and springs for cleaning.

Chapter 18: Troubleshooting

Common Problems and Possible Solutions

Lists common issues and their solutions for instrument operation.

Poor picking results

Troubleshooting steps for poor picking outcomes and parameter checks.

The instrument crashes during destination well inoculation

Checks for plate alignment and lid presence during inoculation.

Appendix A: Replacement Parts and Optional Extras

Appendix B: Technical Specifications

Appendix C: System Diagrams and Dimensions

Appendix D: Electromagnetic Compatibility

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