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Ocean Optics CHEM2000-UV-VIS User Manual

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Operating Manual
and
User’s Guide
CHEM2000 and CHEM2000-UV-VIS
Miniature Fiber Optic Spectrophotometers
Ocean Optics, Inc.
380 Main Street
Dunedin, FL 34698
(727) 733-2447
(727) 733-3962 fax
For the latest information, consult our web site:
www.OceanOptics.com
Or, e-mail our Technical Service Department:
TechSupport@OceanOptics.com
021400
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Ocean Optics CHEM2000-UV-VIS Specifications

General IconGeneral
Wavelength Range200-850 nm
Signal-to-Noise Ratio250:1
A/D Resolution16-bit
Dynamic Range2 x 10^8 (System)
Fiber Optic ConnectorSMA 905
Detector TypeLinear CCD array
Detector2048-element linear silicon CCD array
Data InterfaceUSB 2.0
Stray Light0.05% at 600 nm

Summary

Introduction to Ocean Optics Spectrophotometers

CHEM2000 and CHEM2000-UV-VIS Systems Overview

Overview of the CHEM2000 and CHEM2000-UV-VIS spectrophotometer systems and their components.

Quick Start Guide for System Setup

Initial Setup Steps Overview

High-level overview of connecting the PC2000, installing OOIChem, configuring, and receiving data.

Connect Optics and Receive Initial Data

Steps for connecting optical fibers and performing initial data acquisition.

Installing the PC2000 and OOIChem Software

Finding Available Hardware Resources

Identifying available Base Address and IRQ settings for PC2000 installation in Windows.

Physical PC2000 Installation

Step-by-step guide for physically installing the PC2000 spectrometer A/D card into a computer.

OOIChem Software Installation and Configuration

Procedure for installing and configuring the OOIChem software application.

Using the OOIChem Software

Software Overview and Modes of Operation

Introduction to OOIChem's features, modes, and interface for spectroscopic measurements.

Display Functions and Graph Control

Explanation of screen elements, graph scaling, cursor manipulation, and display options.

Acquisition Parameters and Controls

Settings for data acquisition: integration, averaging, smoothing, reference, and dark scans.

File Menu Functions

Options for saving, opening, and printing spectral data and configuring the printer.

Edit Menu Functions

Functions for clearing data, autoscaling graphs, and managing legends for spectral traces.

Spectrometer Menu Functions

Functions for scanning, selecting wavelengths, calculating calibration curves, and enabling strobe.

Kinetics Configuration

Setting up parameters for kinetic experiments, including duration, sampling interval, and wavelengths.

CHEM2000 System Components

ISS-2 Integrated Sampling System

Description of the ISS-2 light source/sample holder, its parts, operation, and specifications.

CHEM2000-UV-VIS System Components

ISS-UV-VIS Integrated Sampling System

Description of the ISS-UV-VIS light source/sample holder, its parts, operation, and specifications.

Experiment Tutorials

Absorbance Experiments

Step-by-step guide for performing absorbance measurements.

Transmission Experiments

Step-by-step guide for performing transmission measurements.

Reflection Experiments

Guide for performing reflection measurements, noting probe requirements.

Relative Irradiance Experiments

Procedure for measuring relative irradiance, requiring a specific light source.

Concentration Experiments

Steps for concentration measurements and creating a calibration curve using Beer's Law.

Kinetics Experiments

Guide to setting up and running kinetic experiments to monitor spectral changes over time.

Appendix A: PC2000 Configuration Settings

Base Address Settings

Instructions on changing the Base Address settings on the PC2000 A/D converter via dip switches.

Interrupt Request (IRQ) Settings

Instructions on changing the IRQ settings on the PC2000 A/D converter via dip switches.

Appendix B: PC2000 Hardware Connections

H1 Header Pin Assignments

Pinout details for the H1 header on the PC2000 for interconnecting hardware.

J2 Connector Pin Assignments

Pinout details for the J2 D-SUB-15 connector on the PC2000 for various functions.

Appendix C: Wavelength Calibration

Setting Up for Wavelength Calibration

Requirements and preparation steps for calibrating the spectrometer's wavelength.

Performing Wavelength Calibration

Procedure for collecting spectral lines and calculating wavelength calibration coefficients.

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